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Expression data from mouse adrenal gland at 2 month of age

ABSTRACT: V1a vesopressin receptor knockout mouse and wild type mouse were compaired Lipofuscin is an autofluorescent pigment that accumulates in aging cells. We previously found that lipofuscin deposition was accelerated in the adrenal cortex of mice lacking V1a vasopressin receptor gene (V1aKO mice). However, the mechanism underlying V1a receptor- mediated regulation of lipofuscin deposition remains unclear. Upon analysis of the time course of lipofuscin accumulation, we found that autofluorescence in the adrenal cortex first appeared intracellularly and became evident at 2 months of age in male V1aKO mice but not in wild- type mice. At the age of 12 months, the area of the lipofuscin deposit in the adrenal section was 6.8-fold larger in V1aKO mice than that in wild-type mice. Analysis of the expression profiles revealed two upregulated genes, Xaf1 and Serpina1b, and two downregulated genes, Adi1 and Abhd1, in the adrenal cortex of V1aKO mice. The changes in gene expression were confirmed using real-time PCR analysis of adrenal samples from both groups of male mice at 2 months of age. In the adrenocortical Y1 cell line, the V1a receptor was the dominant subtype among the vasopressin/oxytocin receptor family members. Stimulation of Y1 cells with arginine-vasopressin increased intracellular calcium signaling, which was suppressed by a V1a antagonist. Furthermore, in Y1 cells, vasopressin stimulation significantly reduced the Xaf1 transcript levels; this effect has been reported to enhance p53 function and lead to senescence. These data indicate a novel role for the V1a receptor in regulating the aging phenotype in the adrenal cortex.

ORGANISM(S): Mus musculus

PROVIDER: GSE194211 | GEO | 2026/01/01

REPOSITORIES: GEO

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