Global characterization of peripheral B cells in Parkinson’s disease by single-cell RNA and BCR sequencing

ABSTRACT: Fresh blood samples were collected from 8 PD patients and 6 age-matched healthy controls. Then, peripheral blood mononuclear cells (PBMCs) were isolated by Percoll density gradient centrifugation. Next, CD19+ B cells were sorted using fluorescence-activated cell sorting (FACS). Single-cell 5’ gene expression libraries and V(D)J enriched libraries were prepared according to the standard protocols provided by the 10x Genomics Chromium Single Cell Immune Profiling Solution. Finally, single-cell 5’ gene expression and V(D)J libraries were sequenced on Illumina Noveseq 6000, providing 150 bp paired-end reads.

ORGANISM(S): Homo sapiens

PROVIDER: GSE194245 | GEO | 2022/02/10

REPOSITORIES: GEO