A rapid and scalable system for studying gene function in mice using conditional RNA interference

ABSTRACT: Four libraries were analyzed to assess the level and effects of inducing the ectopic expression of a luciferase targeting miRNA in mouse embryonic fibroblasts (MEFs). Here, we find low level, leaky expression in the absence of ROSA-rtTA and doxycyclin. When expression is effectively induced, the Luciferase miRNA is expressed as the 8th most abundant species in the cells. Keyword(s): Epigenetics

ORGANISM(S): Mus musculus

PROVIDER: GSE19476 | GEO | 2010/12/01

SECONDARY ACCESSION(S): PRJNA122263

REPOSITORIES: GEO