Photo-isolation chemistry for high-resolution and deep spatial transcriptome with tissue sections

ABSTRACT: We established a high-depth spatial transcriptomics technology, photo-isolation chemistry (PIC; Honda, et al., Nat Commun, 2021), which is able to isolate gene expression profiles only from photo-irradiated region out of whole tissues. In the original protocol, only the fresh-frozen sections were available, but we have now optimized it applicable to formalin-fixed frozen and formalin-fixed paraffin sections. Here, we provide an updated protocol of PIC.

ORGANISM(S): Mus musculus

PROVIDER: GSE195459 | GEO | 2022/05/04

REPOSITORIES: GEO