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Lariat mapping of a DBR1 CRISPR KO cell line

ABSTRACT: We used CRISPR in HEK293T cells to create two DBR1 knockout cell lines (C19 and C22). After high-throughput sequencing of total RNA extracted from these cells, we performed lariat read mapping using the method described in "Large-scale mapping of branchpoints in human pre-mRNA transcripts in vivo" (Taggart et al, 2012). We found lariats in the two DBR1- cell lines to be ~20-fold enriched relative to the levels observed in the HEK293T control samples.

ORGANISM(S): Homo sapiens

PROVIDER: GSE195586 | GEO | 2022/02/03

REPOSITORIES: GEO

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Lariat mapping of DBR1 KO and DBR1 KO+DBR1 addback cells

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