Transcriptomics

Dataset Information

21

Mouse embryonic kidneys (E13.5): HDAC inhibitor (HDACi) treated vs. Control


ABSTRACT: Transcriptional profiling of cultured CD1 mouse embryonic kidneys (E13.5) comparing HDACi-treated kidneys with control drug-treated kidneys. Studies in our lab showed that pharmacological inhibition of HDAC activity in ex-vivo cultured metanephroi results in extensive defects in kidney development, including impaired UB branching, tubulogenesis, and glomerulogenesis, accompanied by cell cycle arrest and apoptosis.The goal of the microarray analysis was to elucidate the morphogenetic pathways affected by HDACi. Overall design: Two-condition experiment, HDACi-treated E13.5 kidneys (Scriptaid 2.0 μg/ml x 6hrs) vs. Control drug-treated E13.5 kidneys (Nullscript 2.0 μg/ml x 6hrs). Biological replicates: 3 control replicates, 3 HDACi-treated replicates. Two-color Agilent 4x44k chips with dye-swap on 2 of 4 arrays.

INSTRUMENT(S): Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)

ORGANISM(S): Mus musculus  

SUBMITTER: Samir S El-Dahr  

PROVIDER: GSE19581 | GEO | 2010-08-01

SECONDARY ACCESSION(S): PRJNA122353

REPOSITORIES: GEO

Similar Datasets

2010-08-01 | E-GEOD-19581 | ArrayExpress
2012-01-31 | E-GEOD-35432 | ArrayExpress
| PRJNA122353 | ENA
| PRJNA152771 | ENA
2016-06-01 | E-GEOD-58421 | ArrayExpress
| GSE54869 | GEO
2014-08-27 | E-GEOD-60758 | ArrayExpress
2014-08-28 | E-GEOD-60845 | ArrayExpress
2011-02-15 | E-GEOD-27226 | ArrayExpress
2011-02-15 | GSE27226 | GEO