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Microglia P2Y6 receptor signaling regulates a broad range of innate immune responses after TLR engagement

ABSTRACT: We analyzed the transcriptomes of adult primary rhesus macaque microglia that were exposed to 10 µg/mL uLPS, in the absence or presence of 5 µM P2RY6 antagonist MRS2578

ORGANISM(S): Macaca mulatta

PROVIDER: GSE195866 | GEO | 2022/02/04

REPOSITORIES: GEO

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Project description:This study was designed to provide a genome-wide analysis of the effects of luteinizing hormone (LH) ablation/replacement versus steroid ablation/replacement on gene expression in the developed corpus luteum (CL) in primates during the menstrual cycle. Naturally cycling, female rhesus monkeys were left untreated (Control; n = 4) or received one of the following treatments for three days beginning on Day 9 of the luteal phase: daily injection of the gonadotropin-releasing hormone (GnRH) antagonist (Antide; n = 5), Antide + recombinant human LH (A+LH; n = 4), Antide + LH + the 3b-HSD antagonist Trilostane (A+LH+TRL; n = 4), and Antide + LH + TRL + progesterone replacement with a synthetic progestin R5020 (A+LH+TRL+ R5020; n = 5). On Day 12 of the luteal phase, CL were removed and samples of RNA from individual CL were fluorescently labeled and hybridized to Affymetrix™ rhesus macaque total genome microarrays. The greatest number of altered transcripts was associated with the ablation/replacement of LH, while ablation/replacement of progestin affected fewer transcripts. Replacement of LH during Antide treatment restored expression of most transcripts to control levels. Real-time PCR validation of a subset of transcripts revealed that most expression patterns were similar between microarray and real-time PCR. Analysis of protein levels were subsequently determined for 2 of the transcripts differentially expressed by real-time PCR. This is the first genome-wide analysis of LH and steroid regulation of gene transcription in the developed primate CL. Further analysis of novel transcripts identified in this data set can clarify the relative role for LH and steroids in CL maintenance and luteolysis. Keywords: LH/steroid ablation/replacement in primate mid-late luteal phase corpora lutea 22 samples from Rhesus Macaque corpus luteum hybridized to individual Rhesus Affymentrix Gene Chip Arrays. 5 treatment groups, with at least 4 replicates per treatment.

			Action	DRS
		Other
	GSE195866_20220114_P2RY6_analysis.xlsx	Xlsx
	GSE195866_220114_raw_counts.xlsx	Xlsx
	GSE195866_RAW.tar	Raw
	filelist.txt	Txt

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Microglia P2Y6 receptor signaling regulates a broad range of innate immune responses after TLR engagement

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