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Functional definition of thyroid hormone response elements based on a synthetic Starr-seq screen

ABSTRACT: We performed an original protocol called synthetic STARR-seq (PMID: 30427832) in which a library of putative response elements are tested for their capacity to bind thyroid hormone (T3) nuclear receptors and convey T3 transactivation More than 10 000 putative T3 response elements (T3RE) were cloned in the hSTARR-seq-ORI vector with a minimal promoter, in the 3' end of the transcribed sequence. These are variant of the consensus T3RE (5'NGGTCANNNNRGGNNA3') Therefore the most active T3RE are over-represented in the RNA of cells transfected with the plasmid library and treated with T3.

ORGANISM(S): Homo sapiens

PROVIDER: GSE196145 | GEO | 2022/07/06

REPOSITORIES: GEO

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