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Single nucleotide polymorphism determines constitutive versus inducible type VI secretion in Vibrio cholerae

ABSTRACT: In this study, we determined the expression of V. cholerae strain A1552 and its SNP45-converted derivative as well as the expression of strain ATCC25872 and its SNP45-converted derivative.

ORGANISM(S): Vibrio cholerae

PROVIDER: GSE196165 | GEO | 2022/02/04

REPOSITORIES: GEO

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The QstR regulon of Vibrio cholerae

Project description:In this study, we compared the QstR regulon to the TfoX regulon (and TfoY regulon) of V. cholerae in WT strain A1552 and its ΔhapRΔvpsA derivative using RNA-seq to better understand QstR's function.

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The TfoY regulon of Vibrio cholerae.

Project description:In this study, we determined the TfoY regulon of V. cholerae using RNA-seq to better uderstand the protein's function. mRNA profiles of a WT V. cholerae O1 El Tor strain (A1552) and of a TfoY-producing derivative of the WT strain (A1552-TntfoY). 3 independent biological replicates are provided for each bacterial strain. The bacteria were grown to high cell density and in the presence of arabinose (to induce TfoY in strain A1552-TntfoY).

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Project description:These experiments were performed to show a serogroup conversion in Vibrio cholerae from O1 to O139. For this purpose, V. cholerae O1 WT = A1552 was grown on crab shell fragments to induce natural competence for transformation. Purified DNA (2 ug each) from strain VC73-orf6/7-Kan-A was added after 24h and the cells grown further for 24h. The VC73-orf6/7-Kan-A strain is a ATCC25873 derivative (both O37 serogroup) which harbors a Kanamycin cassette in the O37 region (as part of the operon between orf6 and orf7 w/o own promotor) for better selection. Transformants were selected on LB+Kan plates. Three clones were selected from each experiment and analyzed by microarray hybridization (BioPrime. Array CGH Genomic Labeling from Invitrogen). Two microarray replicates were done per clone. Comparison of A1552 versus VC73-orf6/7-Kan-A is shown as control.

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RNAseq analysis of Vibrio cholerae A1552 WT and ∆rvvA (∆VCA0257)

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Comparative genomic hybridization of Vibrio cholerae serogroup conversion from 01 to 0139

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Comparative genomic hybridization of Vibrio cholerae which have converted from serogroup 01 to 0139 (phage selected)

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Intestinal cells infected with Vibrio cholerae

Project description:Each sample was infected with a different strain of V. Cholerae Keywords = Cholerae Keywords: parallel sample

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