Pregestational Diabetes Alters Cardiac changes

ABSTRACT: Objective: To evaluate the altered expression of genes due to hyperglycaemia during foetal development in heart. Methods: To understand the molecular defects in the two-days old neonatal rats, diabetic female rats were bred with healthy male rats and collected two days old hearts from neonates. Heart mRNA profiles of two-day-old neonatal rats were generated by RNA sequencing, in quadruplicate, using Illumina HiSeq. Results: Using an optimized data analysis workflow, we mapped about 27 million sequence reads per sample to the rat genome (build Rnor_6.0) and identified 25456 transcripts in the heart of the neonatal rats. Approximately 4% of the transcripts showed differential expression between the Control and PGDM (pregestational diabetes mellitus) heart, with a fold change ≥2 and p value <0.05. Conclusions: Transcriptomic profiling of the RNA-seq data revealed that several of the altered genes were associated with cardiac pathogenesis in neonatal heart during PGDM.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE196242 | GEO | 2022/07/01

REPOSITORIES: GEO