Transcriptomics

Dataset Information

19

Hyperactivation of PI3K promotes escape from hormone dependence in estrogen receptor-positive breast cancer


ABSTRACT: Hyperactivation of phosphatidylinositol-3 kinase (PI3K) promotes escape from hormone dependence in estrogen receptor-positive breast cancer. A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. We used gene expression microarrays to identify genes and pathways that are commonly dysregulated in ER+ cell lines with acquired hormone-independent growth. MCF-7, ZR75-1, MDA-361, and HCC-1428 ER+, estrogen-responsive breast cancer cells were cultured under hormone-depleted conditions (10% DCC-FBS) for several months until sustainable hormone-independent cell populations emerged. Overall design: Parental and long-term estrogen-deprived (LTED) cells were treated with 10% dextran-coated charcoal-treated fetal bovine serum (DCC-FBS) x 24 hrs prior to RNA harvest for array analysis.

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

ORGANISM(S): Homo sapiens  

SUBMITTER: Todd W Miller   

PROVIDER: GSE19639 | GEO | 2010-06-15

SECONDARY ACCESSION(S): PRJNA122527

REPOSITORIES: GEO

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2015-04-30 | E-GEOD-19639 | ArrayExpress
2014-08-01 | E-GEOD-19639 | ExpressionAtlas
| GSE22533 | GEO
2015-04-30 | E-GEOD-22533 | ArrayExpress
2012-02-10 | GSE27300 | GEO
2014-09-10 | E-GEOD-22533 | ExpressionAtlas
2015-08-20 | E-GEOD-69893 | ArrayExpress
2017-12-11 | PXD004807 | Pride
2016-06-01 | PXD004085 | Pride
2016-06-04 | E-GEOD-80367 | ArrayExpress