Enterococcus Grad-seq and KhpB RIP-seq

ABSTRACT: We investigated the RNA-protein interactome of Enterococcus faecalis V583 and Enterococcus faecium Aus0004 by native gradient fractionation of complexes coupled to RNA-sequencing. Whole bacterial cell lysates were analysed by size and density in a glycerol gradient. At native conditions, RNA-protein complexes stay intact and sediment as a whole. Sedimentation profiles of individual RNAs appear correlated in case of interaction in a complex. The profile of KhpB caught our attention and we determined its RNA interactome by immunoprecipitation that suggests a role at the post-transcriptional level, binding notably several tRNAs, sRNAs, and 3’UTRs.

ORGANISM(S): Enterococcus faecium Aus0004 Enterococcus faecalis V583

PROVIDER: GSE196534 | GEO | 2022/11/03

REPOSITORIES: GEO