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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Adar1 B Cell Conditional Knockout Activated B Cells Transcriptomes

ABSTRACT: Purpose: to compare the gene expression differences between wild-type activated B cells and Adar1 knockout activated B cells Methods: In vitro activated B cells were sorted using a BD FACSAira sorter, and total RNA was extracted using RNeasy Mini Kit (Qiagen) and poly(A) mRNA was isolated with the poly(A) mRNA Magnetic Isolation Module (NEB). Libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) and sequenced on an Illumina Hiseq instrument (Illumina). Sequence reads were filtrated using Cutadapt (V1.9.1), and then mapped to the reference genome sequences (GRCm38.98) using Hisat2 (V2.0.1).

ORGANISM(S): Mus musculus

PROVIDER: GSE196878 | GEO | 2022/06/15

REPOSITORIES: GEO

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