Human lung tissue explants infected with influenza B virus
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ABSTRACT: RNAseq analysis of human lung tissue explants infected with Influenza B virus Results: Differentially expressed genes after infection Project: Hulu_FluB_2022
Project description:Gene expression changes in the blood was studied by RNAseq Results: Strong differential host reponse after infection with influenza virus compared to healthy controls project SIG_2020
Project description:Gene expression changes in the blood was studied by RNAseq; project SIG-2019 Results: Strong differential host reponse after infection with influenza virus compared to healthy controls
Project description:Gene expression changes in the blood was studied by RNAseq; project SIG-2018 Results: Strong differential host reponse after infection with influenza virus compared to healthy controls
Project description:Mouse lung RNAseq after infection with Influenza A virus (H1N1, PR/8/34, mouse-adapted) and Streptococcus pneumoniae (serotype 19F, strain BHN 100) Results: Differentially expressed genes were observed after single and co-infection Project: COST_mouse_2021_lung
Project description:This study investigates the interactions between Influenza A virus (IAV) and lung tissue at the single-cell level using the 10x Genomics single-cell RNA sequencing (scRNAseq) platform. Lung tissue samples from a donor with COPD were infected with IAV, and both infected and uninfected samples were analyzed 24 hours post-infection. Single-cell suspensions from multiple infected and uninfected tissue pieces were pooled and analyzed to identify cell types and their responses. A total of 10 cell types were identified, including various airway epithelial cells and immune cells. Apoptotic T cells, characterized by high mitochondrial DNA content, were excluded from gene expression analyses. The presence of viral mRNA was used to indicate viral transcription activity, although distinguishing between intracellular synthesis and extracellular uptake of viral mRNA remains challenging.
Project description:Gene expression profiles 6 hours post-influenza A virus infection in human monocytes at multiplicities of infection of 10 versus uninfected monocytes
Project description:RNA-seq was performed to study the transcriptomic changes in human lung tissue post infection with either Influenza A virus (IAV), or Pseudomonas aeruginosa (PA) or Mycobacterium bovis (BCG)
Project description:Influenza A virus is a kind of single negative-stranded RNA virus which belongs to the Orthomyxoviridae family. It can cause localized outbreak or worldwide epidemic in a short time for its great contagiosity, fast spread speed and a wide range of host, and H1N1 influenza virus is a strong pathogenic subtype of influenza A virus. Influenza A virus infection has been shown to alter miRNA expression both in cultured cells and in animal models. We used microRNA microarrays to detail the programme of microRNA expression and identified distinct classes of differentially regulated microRNAs during this process.