RNA Sequencing Analyzes Chondrocyte Transcriptomes of Wild-type, miR-26a -/- and Cartilage-specific miR-23a/b clusters Knockout Mice

ABSTRACT: To reveal the potential regulation target genes of miR-26a and miR-23a/b clusters in articular chondrocytes, we performed RNA-seq using RNA samples from cultured chondrocytes, which were primarily isolated from 3-week-old wild-type, miR-26a -/- or miR-23a/b cluster flox/flox;Col2a1-cre mice. Single-read libraries were prepared and sequenced by Illumina Nextseq 500. Proteins from the same sample were extracted for LC-MS/MS analysis. After data processing, differential expressed genes were screened out for exploring potential miRNA regulation targets.

ORGANISM(S): Mus musculus

PROVIDER: GSE197363 | GEO | 2023/01/25

REPOSITORIES: GEO