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Transcriptomic analysis of porcine PSCs (pLCDM and pPSC(KOFL)) and pLCDM_TSC induced from pLCDM

ABSTRACT: To further understand the biological characteristics of porcine pluripotent stem cells (pLCDM) established under LCDM system, transcriptome analyses were performed. The data showed that the biological events related to placental development were found in pLCDMs, while pLCDMs expressed more biological events related to embryo development and organ development of each layer than TE. Transcriptomic analysis demonstrated that pLCDMs expressed higher levels of trophoblast lineage genes than pPSCs (KOFL). Furthermore, we successfully induced trophoblastic-like cells and pLCDM_TSCs with high expression of trophoblast lineage marker genes in vitro using a pure chemical medium. To comprehensively analyze the difference between pLCDM_TSCs and pLCDMs, low input RNA-seq was performed on the cell lines. A total of 3,594 DEGs were found, and 1,606 genes were significantly upregulated in pLCDM_TSCs, including BMP signaling pathway related genes BMPR1A, ID1, and trophoblast lineage marker genes ITGA1, ITGA6, FZD5, etc. It was worth noting that we found 509 highly expressed genes in hTSCs among the genes that were highly expressed in pLCDM_TSCs. Together, these results demonstrated the unique cellular potency of pLCDMs for trophoblast lineage.

ORGANISM(S): Sus scrofa

PROVIDER: GSE197451 | GEO | 2022/05/20

REPOSITORIES: GEO

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