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Human colon cancer cell line HCT116 stably transfected with EPXH2 was constructed and analyzed by RNA sequencing.

ABSTRACT: We analyzed the molecular mechanism of regulation of EPHX2 on colon cancer by constructing colon cancer cell line HCT116 stably transfected with EPHX2.

ORGANISM(S): Homo sapiens

PROVIDER: GSE197535 | GEO | 2022/03/02

REPOSITORIES: GEO

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Human colon cancer cell line HCT116 stably transfected with EPXH2 was constructed and analyzed by RNA sequencing.

Project description:Human colon cancer cell line HCT116 stably transfected with EPXH2 was constructed and analyzed by RNA sequencing.

| PRJNA810849 | ENA

Epigenome analysis of HDAC1 stably knocked down HCT116 cells and HCT116 cells stably expressing UHRF1 mutant

Project description:Epigenome analysis of HDAC1 stably knocked down HCT116 cells and HCT116 cells stably expressing UHRF1 mutant

| PRJNA637512 | ENA

Analysis of microRNA expression through microarray scan in HCT116 human colon cancer cells with or without METTL14 overexpression

Project description:To reveal the changes of miRNAs levels in HCT116 cells with METTL14 overexpression, we employed miRNA microarray expression profiling in HCT116 human colon cancer cells stably expressing control vector or METTL14.

2023-03-27 | GSE186790 | GEO

Expression profiling of HCT116 cells transfected with shCTRL or shKSR1

Project description:We engineered HCT116 cells by transfecting a non targeting control hairpin or 2 different short hairpin against KSR1 to knockdown KSR1 and microarrays were performed on non-transfected controls, non-targeting shRNA controls, shRNA #1 targeting KSR1, and shRNA #2 targeting KSR1. We used microarray for molecular profiling of colon cancer cells in presence or in absence of KSR1, an important scaffold of Raf/MEK/ERK pathway and identify genes which are regulated by KSR1 Cell lines were measured in as single biological replicates 1. HCT116 untransfected 2 HCT116 + shCTRL 3.HCT116 + shKSR1#1 4. 3.HCT116 + shKSR1#2

2015-01-28 | E-GEOD-65351 | biostudies-arrayexpress

Human colon cancer HT-29 and LS174T cells: stably transfected with shRNA-Ascl2/EGFP vs. stably transfected with shRNA-Control/EGFP

Project description:Achaete scute-like 2 (Ascl2), a basic helix-loop-helix (bHLH) transcription factor, controls the fate of intestinal stem cells. However, the role of Ascl2 in colon cancer progenitor cells remains unknown. The cell lines HT-29 (47.5-95% of CD133+ population) and LS174T (0.45% of CD133+ population) were chosen for functional evaluation of Ascl2 in colon cancer progenitor cells after gene knockdown by RNA interference. The microRNA (miRNA) microarrays identified 26 two-fold up-regulated miRNAs and 58 two-fold down-regulated miRNAs in shRNA-Ascl2/HT-29 cells and 178 two-fold up-regulated miRNAs and 172 two-fold down-regulated miRNAs in shRNA-Ascl2/LS174T cells. Two-condition experiment: shRNA-Ascl2/HT-29 cells vs. shRNA-Ctr/HT-29 cells, and shRNA-Ascl2/LS174T cells vs. shRNA-Ctr/LS174T cells. Biological replicates: 1 HT-29 cells stably transfected with shRNA-Ascl2/EGFP, 1 LS174T cells stably transfected with shRNA-Ascl2/EGFP, 1 HT-29 cells stably transfected with shRNA-Control/EGFP, and 1 LS174T cells stably transfected with shRNA-Control/EGFP, independently grown and harvested. One replicate per array.

2012-01-07 | E-GEOD-34926 | biostudies-arrayexpress

Epigenome analyses of HCT116 cells stably expressing UHRF1 mutant [Illumina EPIC]

Project description:Epigenome analyses of HCT116 cells stably expressing UHRF1 mutant [Illumina EPIC]

| PRJNA637514 | ENA

Epigenome analysis of HDAC1 stably knocked down HCT116 cell [Illumina EPIC]

Project description:Epigenome analysis of HDAC1 stably knocked down HCT116 cell [Illumina EPIC]

| PRJNA637513 | ENA

High-throughput RNA sequencing analysis of human colon cancer cell line HCT116 transfected with LINC00152 siRNA.

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are using RNA sequencing analysis to evaluate the effects of si-LINC00152 on the mRNA of human colon cancer cell line HCT116. Methods: Human colon cancer cell line HCT116 was transfected with a control non-targeting siRNA to cells or transfected with siRNA targeting LINC00152 for 36 hours in DMEM medium (with 10% serum). Total RNA were extracted and detected by Illumina high-throughput RNA sequencing data analysis. 2 independent biological replicates were plated, transfected in parallel for each control and siRNA. Results: Log-fold changes of up- or down-regulated mRNAs between the control and experiment group were selected with a significance threshold of p<0.05. There are 90 mRNAs were up-regulated and 159 were down-regulated in “si-LINC00152” group comparing to “control” group. Conclusions: Our study describes the mRNA changes of human colon cancer cell line HCT116 transfected with LINC00152 siRNA.

2020-02-18 | GSE132519 | GEO

Identification of genes differentially expressed between control and EHF siRNA transfected HCT116 cells

Project description:we found that EHF allowed colon tumor cells to escape p53-mediated apoptosis.We performed microarray analysis to find the target genes of EHF. A colorecal cancer cell line HCT116 was transfected with an siRNA targeting EHF or negative control.

2010-07-29 | E-GEOD-22265 | biostudies-arrayexpress

Human colon cancer SW480 cells: stably transfected with vector control and LRP16 treated with or without etoposide

Project description:Transcriptional profiling of human colon cancer SW480 cells comparing control untreated SW480 cells with cells stably transfected with LRP16 treated with or without etoposide (50 μM) for the indicated periods (0,1hour, 3hours).An exploratory microarray analysis was performed with mRNA extracted from clutured SW480 cells transfected with LRP16 or control plasmid that were treated with or without etoposide. Total RNA of colon cancer cells stably transfected with vector control and LRP16 treated with or without etoposide (50 μM) for the indicated periods was isolated and purified using RNeasy Kit (Qiagen, Hilden, Germany). Integrity of RNA was assessed by using an Agilent BioAnalyser 2100 (Agilent Technologies).

2017-09-22 | GSE93625 | GEO

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