RAINBOW-seq to capture the spatial transcriptome of bacterial biofilm

ABSTRACT: We developed a spatially resolved method to profile the spatial transcriptome of biofilm. In detail, we used fluorescent dyes to label the different regions of biofilm cultured in a microfluidic chip. After staining, the bacterial cells in biofilm were sorted into relevant bins according to their spatial information marked by the fluorescent pattern. Finally, miniBac-seq (RNA-seq) method was applied to capture the transcriptome of each bin.

ORGANISM(S): Escherichia coli BW25113

PROVIDER: GSE197541 | GEO | 2023/01/01

REPOSITORIES: GEO