Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mycoplasma gallisepticum: Rlow vs. F

ABSTRACT: Mycoplasma gallisepticum transcriptome comparison between in vitro grown cultures of strains Rlow and F utilizing oligo DNA microarrays.

ORGANISM(S): Mycoplasmoides gallisepticum Mycoplasmoides gallisepticum str. R

PROVIDER: GSE19755 | GEO | 2010/01/07

SECONDARY ACCESSION(S): PRJNA122175

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Mycoplasma gallisepticum: Rlow vs. F

Project description:Mycoplasma gallisepticum transcriptome comparison between in vitro grown cultures of strains Rlow and F utilizing oligo DNA microarrays. Two-condition experiment, Rlow vs. F strain cells. Biological replicates: 3. 1 technical replicate per biological replicate which includes a dye swap.

2010-04-22 | E-GEOD-19755 | biostudies-arrayexpress

Transcription profiling of different state of Mycoplasma gallisepticum

Project description:Mycoplasma gallisepticum is a convenient model object for studying the regulation of transcription because it has a reduced genome, lack of cell wall and many metabolic pathways, and also easy to culture and non-pathogenic to humans. For rapid investigation of gene expression we developed microarray design including 3 366 probes for 678 genes. They included 665 protein coding sequences and 13 antisense RNAs from 816 genes and 17 ncRNAs present in Mycoplasma gallisepticum. This work was carried out transcriptomic profiling for different types of effects on the expression of genes of Mycoplasma gallisepticum: 1) genetic knock-out mutants; 2) cell culture exposed to sublethal concentrations of antibiotics; and 3) well-characterized heat stress effect. The study was performed on Agilent one-color microarray with custom design and random-T7 polymerase primer for cDNA synthesis. Using set of different probes for each gene or ncRNA allows to increase accuracy of gene expression quality.

2016-08-18 | GSE85777 | GEO

Mycoplasma gallisepticum WhiA knockdown and overexpression

Project description:Proteomes of Mycoplasma gallisepticum strains with overexpression and knockdown of WhiA transcription factor. The overexpression was introduced on a transposon vector carrying M. gallisepticum whiA gene with strong constitutive promoter and strong SD sequence. The knockdown was made by CRISPRi. dCas9 protein and sgRNA against whiA gene were introduced on a transposon vector.

2021-06-25 | PXD026928 | Pride

Mycoplasma gallisepticum

Project description:Mycoplasma gallisepticum: Rlow vs. F

| PRJNA122175 | ENA

Mycoplasma gallisepticum

Project description:Comparative Genomic Hybridizations of Mycoplasma gallisepticum Vaccine strains and strain Rlow

| PRJNA122169 | ENA

Comparative Genomic Hybridizations of Mycoplasma gallisepticum Vaccine strains and strain Rlow

Project description:Genomic content of Vaccine strains were probed against the known sequence of the virulent strain Rlow of M. gallisepticum to identify divergent or absent genes in the attenuated strains. Genomic DNA was extracted from in vitro grown cultures of M. gallisepticum strains Rlow, F, ts-11, and 6/85 and three samples from each were selected for hybirdization to oligonucleotide microarrays using standard methods from the Bioprime CGH kit. All samples were labeled with Cy3 dye and scanned at a PMT gain of 700 using a GenePix 4000B scanner. Features are duplicated on the slides and data were averaged between duplicate features on each slide. Median signal intensities were averaged between samples of the same strain and each feature from each vaccine strain was compared to Rlow. Features exhibiting four-fold or less hybridization in the vaccine strains were considered divergent or absent.

2010-05-16 | E-GEOD-19629 | biostudies-arrayexpress

Mycoplasma gallisepticum

Project description:Mycoplasma gallisepticum Genome sequencing and assembly

| PRJNA445284 | ENA

Mycoplasma gallisepticum

Project description:Mycoplasma gallisepticum Genome sequencing and assembly

| PRJNA325637 | ENA

Mycoplasma gallisepticum Rlow attached to MRC-5 cells microarray

Project description:The goal of this study was to identify genes important to the interaction of Mycoplasma gallisepticum with host cells in an in vitro system. An interaction time of one hour was chosen since it is less than a generation time and thus all changes can be attributed to transcriptional changes. A total of 60 transcripts were determined to be significantly up- or down-regulated under these conditions, including several hypothetical genes. Also several metabolism-related genes and ATP synthase genes were significantly down-regulated. Keywords: transcriptional response to host cells

2007-05-20 | GSE6717 | GEO

Comparative Genomic Hybridizations of Mycoplasma gallisepticum Vaccine strains and strain Rlow

2009-12-24 | GSE19629 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data