Transcriptomics

Dataset Information

20

TTHB212 (anti-sigmaE)-deficient Thermus thermophilus HB8 strain: time course


ABSTRACT: We observed the expression profile of the total mRNA of TTHB212-deficient of Thermus thermophilus HB8 strain grown in a rich medium at 70°C for 7 and 8 hours Overall design: TTHB212-deficient T. thermophilus HB8 cells were each pre-cultured at 70°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70°C. Cells were collected after 7 or 8 h, respectively, and then, the expression of each mRNA was analyzed.

INSTRUMENT(S): [TTHB8401a520105F] Riken Thermus thermophilus HB8 4K mRNA custom array, library Rev.2

ORGANISM(S): Thermus thermophilus HB8  

SUBMITTER: Akeo Shinkai  

PROVIDER: GSE19758 | GEO | 2010-10-15

SECONDARY ACCESSION(S): PRJNA129747

REPOSITORIES: GEO

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