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Functional crosstalk between the cohesin loader and a chromatin remodeler

ABSTRACT: The Scc2-Scc4 complex interacts with a conserved RSC ATPase motor module. The cohesin loader enhances RSC remodeling activity in vitro and promoter nucleosome eviction in vivo.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE197657 | GEO | 2022/03/01

REPOSITORIES: GEO

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Functional crosstalk between the cohesin loader and chromatin remodelers

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The Scc2NIPBL/Scc4MAU2 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions [microarray]

Project description:The Scc2/Scc4 complex binds to broad nucleosome-free regions in the promoters of highly expressed genes. The cohesin loader is recruited to these sites by the RSC chromatin remodeling complex Total RNA was isolated from wt, scc2-4 and sth1-3 cells at 25Â°C and 37Â°C. dscDNA was obtained using the IVT system (Invitrogen) and hybridized to Affymetrix Yeast Genome 2.0 Arrays to analyze the expression profile of wt, scc2-4 and sth1-3 cells.

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