N6-adenine DNA-immunoprecipitation-based genomic profiling in eukaryotes

ABSTRACT: The detection of low-abundance DNA N6-methyladenine (DNA-m6A) remains challenging, limiting our understanding of this novel base in eukaryotes. To address this, we introduce an approach for systematically validating the selectivity and sensitivity of antibody-based DNA-m6A methods, revealing most commercial antibodies as poorly selective towards DNA-m6A. Finally, we validate selective anti-DNA-m6A antibodies with sensitivity <2 ppm, and expose distinct pathways mediating endogenous DNA-m6A in C. reinhardtii, A. thaliana, and D. melanogaster.

ORGANISM(S): Arabidopsis thaliana Chlamydomonas reinhardtii Drosophila melanogaster

PROVIDER: GSE197747 | GEO | 2023/02/02

REPOSITORIES: GEO