Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq in H1299 cell lines.

ABSTRACT: Purpose: The goals of this study are to invsetigate the primary biological process of CLCN3. Methods: Briefly, samples (H1299 cells transfected with control or CLCN3 shRNA) were used to extract total RNAs for quality control and RNA-seq loading samples. RNA-seq was performed as DGE on an Illumia HiSeq platform and 50 bp paired-end reads were generated (BGI Co. Ltd.). Results: Locomotion and growth were identified as the biological functions of CLCN3 Conclusions: RNA-seq in H1299 cell lines revealed that CLCN3 mainly regulated locomotion and growth.

ORGANISM(S): Homo sapiens

PROVIDER: GSE197845 | GEO | 2022/03/06

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq in H1299 cell lines.

Project description:Purpose: The goals of this study are to invsetigate the primary biological process of HNRNPK. Methods: Briefly, samples (H1299 cells transfected with control or HNRNPK shRNA) were used to extract total RNAs for quality control and RNA-seq loading samples. RNA-seq was performed as DGE on an Illumia HiSeq platform and 50 bp paired-end reads were generated (BGI Co. Ltd.). Results: Gene Ontology (GO) analysis revealed that cell proliferation and migration were identified as the biological functions of HNRNPK Conclusions: RNA-seq in H1299 cell lines revealed that HNRNPK mainly regulated cell proliferation and migration.

2022-03-09 | GSE197946 | GEO

RNA-seq in H1299 cell lines

Project description:Purpose: The goals of this study are to investigate the primary biological process of HNRNPC. Methods: Briefly, samples (H1299 cells transfected with control or HNRNPC shRNA) were used to extract total RNAs for quality control and RNA-seq loading samples. RNA-seq was performed as DGE on an Illumia HiSeq platform and 50 bp paired-end reads were generated (BGI Co. Ltd.). Results: RNA-seq in H1299 cell lines revealed that HNRNPC mainly regulated cell growth, cell migration, extracellular matrix organization, angiogenesis, collagen fibril organization, and T cell mediated cytotoxicity. Conclusions: HNRNPC mainly regulated cell growth, cell migration, extracellular matrix organization, angiogenesis, collagen fibril organization, and T cell mediated cytotoxicity.

2022-03-30 | GSE199483 | GEO

RNA-seq (CAFs stimulated with control or HNRNPK knockdown supernatants)

Project description:Purpose: The goals of this study are to invsetigate the primary signaling pathway enriched in CAFs after stimulation of LUAD supernatants. Methods: Briefly, the supernatants (HNRNPK knockdown and control) of H1299 cells were incubated with CAFs for 24h. Then the CAFs were used to extract total RNAs for quality control and RNA-seq loading samples. RNA-seq was performed as DGE on an Illumia HiSeq platform and 50 bp paired-end reads were generated (BGI Co. Ltd.). Results: Gene Ontology (GO) analysis revealed that cell proliferation and migration were identified as the biological functions of HNRNPK Conclusions: In CAFs, we confirmed that the PI3K-AKT signaling pathway was significantly enriched after stimulation of H1299 supernatants.

2022-03-02 | GSE197540 | GEO

RNA-seq in HCT116 cell lines

Project description:Purpose: The goals of this study are to invsetigate the primary biological process of hnRNPK. Methods: Briefly, samples (HCT116 cells transfected with control or hnRNPK shRNA) were used to extract total RNAs for quality control and RNA-seq loading samples. RNA-seq was performed as DGE on an Illumia HiSeq platform and 50 bp paired-end reads were generated (BGI Co. Ltd.). Results: Regulation of transcription by RNA polymerase II was identified as the primary biological process according to the gene ontology (GO) analysis. Regulation of transcription by RNA polymerase II and translation by ribosome were identified as the primary biological processes according to the gene set enrichment analysis (GSEA). Conclusions: RNA-seq in HCT116 cell lines revealed that hnRNPK mainly regulated cellular transcription and translation.

2021-10-24 | GSE186371 | GEO

RNA-seq in FHC cell lines.

Project description:Purpose: The goals of this study are to invsetigate the primary biological process of hnRNPK. Methods: Samples (FHC cells transfected with control or hnRNPK shRNA) were used to extract total RNAs for quality control and RNA-seq loading samples. RNA-seq was performed as DGE on an Illumia HiSeq platform and 50 bp paired-end reads were generated. Results: In FHC, metabolism was regulated hnRNPK. Conclusions: RNA-seq in FHC cell lines revealed that hnRNPK mainly regulated metabolism.

2022-03-18 | GSE198445 | GEO

HBx increases chromatin accessibility and ETV4 expression to regulate Dishevelled-2 and promote HCC progression

Project description:Purpose: The goals of this study are to invsetigate the primary biological process of HBx. Methods: Briefly, samples (L02 cells transfected with pcDNA4.0-vector or pcDNA4.0-HBx) were used to extract total RNAs for quality control and RNA-seq loading samples. RNA-seq was performed as DGE on an Illumina platform and 50 bp paired-end reads were generated (Guangzhou Epibiotek Co.,Ltd.). Results: Negative regulation of transferase activity was identified as the primary biological process according to the gene ontology (GO) analysis. TNF signaling pathway was identified as the primary biological processes according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis . DNA binding transcription factor activity was identified as the primary biological processes according to the gene set enrichment analysis (GSEA). Conclusions: RNA-seq in L02 cell lines revealed that HBx mainly regulated the DNA binding transcription factor activity.

2021-11-01 | GSE186862 | GEO

Gene expression data from H1299 cells

Project description:MiR-138 has a variety of biological functions because of its capacity to act on different target genes in various cells and tissues; however, the targets of miR-138 in human non-small cell lung cancer cell line H1299 cannot be determined by bioinformatics alone. Thus, H1299 cells overexpressing miR-138 in H1299 cells were subjected to microarray analysis to analyse the differences of gene expression.

2016-05-06 | GSE69482 | GEO

ZBTB11 and SET regulates genes in NCI-H1299

Project description:To determined ZBTB11 and SET regulates genes in NCI-H1299, we esteblished NCI-H1299 cell lines in which ZBTB11 and SET has been knocked down by si-RNA. We then conducted differential expressed genes analysis using data generated form RNA-seq of H1299 cell lines at the condition of two genes knocked down.

2023-12-21 | GSE206957 | GEO

Whole genome gene expression profiles of H1299-shNaa10 and H1299-shRNA negative control

Project description:Whole genome gene expression profiles of H1299-shNaa10 and H1299-shRNA negative control

2016-10-07 | GSE87674 | GEO

MicroRNA expression data from H1299 cells

Project description:We explored the biological effects of AGO2 downregulation on the expression of miRNAs in human NSCLC cells. Using AGO2 siRNA as a positive control, we overexpressed miR-138 in H1299 cells to observe changes in the miRNA profile using an miRNA microarray assay.

2015-06-05 | GSE69561 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data