Bulk RNA-seq of Normal and Doxorubicin-Induced Senescent HCA2 cells

ABSTRACT: Purpose: Use RNA-seq strategy to characterize the PD-L1 correlated genes in sorted PD-L1 negative and positive doxorubicin-induced senescent and normal human foreskin fibroblasts HCA2 cells to identify the upstream transcriptional network for regulating heterogeneous PD-L1 expression in senescent cells. Methods: Early passaged normal HCA2 cells and senescent HCA2 cells induced by 24 hours of 100 nM doxorubicin treatment were utilized for the preparation of bulk RNA-seq libraries. Among the senescent cells, PD-L1 positive and negative cells were separated by FACSorting.

ORGANISM(S): Homo sapiens

PROVIDER: GSE198396 | GEO | 2022/08/27

REPOSITORIES: GEO