Transcriptomics,Genomics

Dataset Information

0

Transcriptome profiling reveals novel gene networks regulating tooth regeneration in sharks


ABSTRACT: The dentition of elasmobranchs (sharks, skates and rays) is uniquely productive, capable of both rapid and continuous, lifelong regeneration. Elasmobranchs represent an important group of vertebrates with a deep evolutionary history, possessing several ancient and basal characters, i.e., the continuously regenerative dentition from a specialized dental lamina. The dental lamina is an expanded component of the oral epithelia that is responsible for initiating and producing new teeth among all toothed-vertebrates. In sharks, this dynamic epithelial unit is permanent and continuous – meaning it extends to cover the entirety of each jaw (jaw-wide) and develops early during embryogenesis and retained to produce teeth for the life of the shark. It is rare for a truly embryonic vertebrate tissue to be retained for its original function for the life of the organism. The dental lamina in sharks is unique and houses teeth in a developmental series from the deepest part, where teeth are initiated, through stages of tooth development in the form of a related, family of teeth to eruption and functionality of the advanced teeth at the jaw margin. How teeth are made and regenerated is an important question in vertebrate biology; here we investigated this question in the small spotted catshark (Scyliorhinus canicula), a new model in the field of developmental biology. Specifically, we divided the shark dental lamina into stage-compartments as follows: (i) the initiation site – the successional lamina (SL); (ii) the early developing teeth (ET); (iii) the late stage developing teeth (LT); (iv) the tooth-taste junction between the superficial oral and dental epithelium at the jaw margin that separates the taste territory and the dental lamina proper (TTJ); and basi-hyal oral epithelium that is strictly non-dental and only contains taste buds (BHTB). These 5 compartments each house both a shared and unique signature of gene transcripts. This study aims to understand the transcriptomic basis of continuous tooth regeneration in the shark. In this study we combine X-ray computed tomography, classic histology, insitu hybridization, immunohistochemistry, and functional assays of novel markers, and de novo and genome guided transcriptome assemblies for each of these 5 dental lamina compartments of the hatchling (stage 34) catshark (S. canicula). Overall design: We produced both a de novo and genome-guided transcriptome assembly and expression analysis of the 5 dental lamina compartments (SL, ET, LT, TTJ and BHTB) of developing dentition in the hatching-stage (stage 34) small-spotted catshark (Scyliorhinus canicula).

INSTRUMENT(S): Illumina HiSeq 2000 (Scyliorhinus canicula)

ORGANISM(S): Scyliorhinus Canicula

SUBMITTER: Katherine James  

PROVIDER: GSE198580 | GEO | 2022-07-01

REPOSITORIES: GEO

Similar Datasets

| S-EPMC5187730 | BioStudies
| S-EPMC4640805 | BioStudies
| S-EPMC4444311 | BioStudies
| S-EPMC6731641 | BioStudies
| S-EPMC4614774 | BioStudies
| S-EPMC2660956 | BioStudies
| S-EPMC3760860 | BioStudies
| S-EPMC2637924 | BioStudies
| S-EPMC9296035 | BioStudies
| S-EPMC2843623 | BioStudies