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Cortical and hippocampal gene expression after transcranial focal stimulation in healthy rats

ABSTRACT: Transcranial Electrical Stimulation has therapeutic effects on different diseases, including epilepsy. Nevertheless, previous evidence also suggest that TFS could modify protein expression in the healthy brain of rats. We aimed to screen genetic expression in the cerebral cortex and the hippocampus of healthy rats to identify potential targets of therapeutic or adverse effects of TFS.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE198994 | GEO | 2024/03/30

REPOSITORIES: GEO

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Cortical and hippocampal gene expression after transcranial focal stimulation and a single seizure in rats

Project description:Transcranial Electrical Stimulation has therapeutic effects on different diseases, including epilepsy. Particularly, Transcranial Focal Stimulation (TFS) has anticonvulsive and antiepileptogenic effects. Nevertheless, the mechanisms and therapeutic targets that induced these effects remain ellusive. We aimed to screen the genetic expression in the cerebral cortex and the hippocampus of rats after a single seizure and TFS to identify potential targets.

2024-03-30 | GSE199183 | GEO

Transcranial current stimulation in the sensorimotor cortex of rats alters the expression of immune-mediating genes

Project description:Transcranial direct current stimulation (tDCS) has been applied in experimental and clinical settings for more than twenty years and may facilitate rehabilitation after stroke as suggested by clinical data (Hummel et al. 2005, Sparing et al. 2009). Intriguingly, tDCS evokes various cellular effects exceeding its primary neurophysiological actions: We previously described subacute effects of tDCS on immune- and stem cells in the rat brain (Rueger et al. 2012, Keuters et al. 2015, Pikhovych et al. 2016, Braun et al. 2016). However, the effects of tDCS on immune-mediating genes have not yet been investigated. To investigate the more immediate effects of tDCS regulating those cellular responses, we treated rats with a single session of either anodal or cathodal tDCS, and analyzed the gene expression by microarray; sham-stimulated rats served as control. We confirmed the effects of gene upregulation by immunohistochemistry at the protein level. We here report for the first time that acute anodal transcranial direct current stimulation enhanced the expression of several genes coding for MHC-I, affecting inflammation and synaptic plasticity (Neumann et al. 1995, Shatz et al. 2009). Moreover, cathodal tDCS increased expression of the gene encoding for the immunoregulatory protein Osteopontin. Osteopontin has beneficial effects on neural stem cells and microglia after brain damage such as stroke (Rabenstein et al. 2015, Rabenstein et al. 2016, Ladwig et al. 2017, Rogall et al. 2017). Overall, our data indicate that a specific modulation of neuroinflammatory processes by non-invasive brain stimulation constitutes a promising therapeutic option with immediate translational relevance.

2019-10-31 | E-MTAB-8318 | biostudies-arrayexpress

Transcriptional changes in the rat brain induced by repetitive transcranial magnetic stimulation (rTMS)_IN_VIVO

Project description:Transcranial Magnetic Stimulation (TMS) is a noninvasive technique that uses pulsed magnetic fields to affect the physiology of the brain and central nervous system. Repetitive TMS (rTMS) has been used in the study and treatment of neurological conditions including major depression, stroke, epilepsy, schizophrenia, multiple sclerosis, Parkinson’s, and Alzheimer’s disease, as well as other, non-neurological disorders. Among the effects of rTMS, low-frequency protocols (≤ 1Hz) are generally understood to result in cortical inhibition, whereas high-frequency protocols (≥ 3Hz) increase cortical excitability. However, the complex molecular basis of rTMS is largely unexplored. Using three complementary rat models, in vitro, ex vivo, and in vivo, we show complex patterns of hippocampal and neocortical transcriptional response to stimulation in glutamatergic and GABAergic signaling pathways, as well as multiple inflammatory pathways, among others. This broad-based molecular survey helps provide a foundation to tease out the complex molecular mechanisms of the effects of rTMS.

2023-05-30 | GSE230149 | GEO

Transcriptional changes in the rat brain induced by repetitive transcranial magnetic stimulation (rTMS)_EX_VIVO

2023-05-30 | GSE230148 | GEO

Transcriptional changes in the rat brain induced by repetitive transcranial magnetic stimulation (rTMS)_IN_VITRO

2023-05-30 | GSE230147 | GEO

Alterations in mRNA levels in the dentate gyrus in epileptic rats

Project description:Analysis of the dentate gyrus of amygdala electrical stimulation model of temporal lobe epilepsy. Results provide insight into the molecular mechanism underlying epileptogenesis. This study was designed to estimate changes in gene expression levels after 7 and 30 days after electrical stimulation of amygdala as a model of temporal lobe epilepsy. The advantage of this study is time matched control (sham operated animals sacrificed at the same age as stimulated animals).

2013-08-14 | E-GEOD-49849 | biostudies-arrayexpress

Alterations in miRNA levels in the dentate gyrus in epileptic rats

Project description:Analysis of the dentate gyrus of amygdala electrical stimulation model of temporal lobe epilepsy. Results provide insight into the molecular mechanism underlying epileptogenesis. This study was designed to estimate changes in miRNA expression levels after 7, 14, 30 and 90 days after electrical stimulation of amygdala as a model of temporal lobe epilepsy. The advantage of this study is time matched control (sham operated animals sacrificed at the same age as stimulated animals).

2013-08-14 | E-GEOD-49850 | biostudies-arrayexpress

High-Frequency Repetitive Transcranial Magnetic Stimulation (rTMS) Protects Against Ischemic Stroke by Inhibiting M1 Microglia Polarization through Let-7b-5p/HMGA2/NF-κB Signaling Pathway

Project description:Modulating M1/M2 polarization is a potential therapy for treating ischemic stroke. Repetitive transcranial magnetic stimulation (rTMS) held the capacity to regulate astrocytic polarization, but little is known about rTMS effects on microglia. Therefore, the present study aimed to investigate whether and how rTMS influence microglia polarization in ischemic stroke models. The 10-Hz rTMS was applied to transient middle cerebral artery occlusion (MCAO) rats and oxygen and glucose deprivation/ reoxygenation injured BV2 cells. Western blot, immunofluorescence and ELISA were used to detect M1/M2 markers. High-throughput sequencing, RT-PCR and FISH staining were adopted to test microRNA changes. The 10-Hz rTMS inhibited ischemia/reperfusion induced M1 microglia and significantly increased let-7b-5p. HMGA2 was proved to be the target protein of let-7b-5p and its downstream NF-κB signaling pathway were inhibited by rTMS. Microglia culture medium (MCM) collected from rTMS treated microglia had low TNF-α but high IL-10 concentration, leading to reduced neural death, minor ischemic volumes and improved functional recovery of MCAO animals. However, knockdown of let-7b-5p by antagomir reversed rTMS effects on microglia. In conclusion, high-frequency rTMS could improve functional recovery through inhibiting M1 microglia polarization via regulating let-7b-5p/HMGA2/NF-κB signaling pathway in cerebral ischemic stroke models.

2022-08-10 | GSE192910 | GEO

Transcriptomics analysis of the effects of electrical stimulation on rat denervated gastrocnemius muscle

Project description:We report the application of RNA sequencing technology for transcriptomics analysis of the effects of electrical stimulation on rat denervated gastrocnemius muscle. Muscle samples were selected for gene expression analyses，corresponding to the following categories: Sham operation rats, (SHAM, n = 3), sciatic nerve injury rats (DN, n = 3), and sciatic nerve injury with electrical stimulation (DN-SM, n = 3). A total of 14,334 genes (DEGs) were detected in this study.

2020-07-01 | GSE117440 | GEO

Alterations in miRNA levels in the dentate gyrus in epileptic rats

2013-08-14 | GSE49850 | GEO

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