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ATAC-seq chromatin accessibility profiling in pancreatic islets isolated from Arid1aFl/Fl ; MIP-rtTA control and Arid1aFl/Fl; MIP-rtTA; TRE-Cre mice (Arid1a βKO) with/without pancreatectomy.

ABSTRACT: To determine how ARID1A loss might regulate genes that were preferentially regulated on the gene expression level, we performed an assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) on islets isolated from control and Arid1a β-cell knockout (βKO) mice at baseline and 6 days after 50% pancreatectomy (PPx).

ORGANISM(S): Mus musculus

PROVIDER: GSE199186 | GEO | 2022/03/22

REPOSITORIES: GEO

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ATAC-seq chromatin accessibility profiling in pancreatic islets isolated from Arid1aFl/Fl ; MIP-rtTA control and Arid1aFl/Fl; MIP-rtTA; TRE-Cre mice (Arid1a βKO) with/without pancreatectomy

Project description:To determine how ARID1A loss might regulate genes that were preferentially regulated on the gene expression level, we performed an assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) on islets isolated from control and Arid1a β-cell knockout (βKO) mice at baseline and 6 days after 50% pancreatectomy (PPx).

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| PRJNA827843 | ENA

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Transcription profiling of mouse islet growth after partial pancreatectomy and exendin-4 Treatment

Project description:Diabetes mellitus results from an inadequately functioning beta-cell mass. In the adult pancreas, beta-cell mass is dynamic, increasing to meet metabolic demands and decreasing with metabolic or injury insults. Exendin-4 (Ex-4) is a glucagon-like peptide-1 receptor agonist that augments beta-cell mass by increasing beta-cell neogenesis and proliferation and by reducing apoptosis. We utilized a cDNA microarray approach to identify genes that are differentially regulated during islet growth after Ex-4 treatment or a partial pancreatectomy (Ppx). Mice underwent 50% Ppx or sham operation and received Ex-4 or vehicle every 24 hours. cDNA prepared from total pancreatic RNA isolated at 12, 24 and 48 hrs after surgery was hybridized to the PancChip 4.0 microarray.

2005-12-01 | E-CBIL-5 | biostudies-arrayexpress

RNA-Seq in pancreatic islets isolated from Arid1aFl/Fl control and Arid1aFl/Fl; UBC-CreERT2 mice (Arid1a KO) with/without pancreatectomy.

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