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Dual RNA-seq of Trichophyton mentagrophytes complex

ABSTRACT: We report the application of dual RNA-sequencing technology for high-throughput profiling of histone modifications in HaCat cells and Trichophyton mentagrophytes complex.For co-culture assays, a ratio of 2.5×105 cells/mL of keratinocytes to 2.5×105 conidia/mL of T. mentagrophytes, T. interdigitale, and T. tonsurans solution were used (MOI=1). The experiment was carried out for 24 h in a humidified incubator maintained at 37 ºC . We used dual RNA-seq to study the different host immune responses against the T. mentagrophytes complex and we the transcriptional profiles of differentially expressed genes in dermatophytes.

ORGANISM(S): Trichophyton interdigitale Homo sapiens Trichophyton mentagrophytes Trichophyton tonsurans

PROVIDER: GSE199307 | GEO | 2022/08/19

REPOSITORIES: GEO

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