Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell RNA sequencing analysis of mouse normal brain and GBM tumors

ABSTRACT: To investigate the transcriotome in glioblastoma tumor and normal brain on a single cell level, we performed RNA sequcening analysis with single cells derived from mouse normal brain tissue or GBM tumor tissue. Particularly, vascular endothelial cells (ECs) were traced using a Cdh5-Cre;LSL-tdTomato system.

ORGANISM(S): Mus musculus

PROVIDER: GSE199379 | GEO | 2023/02/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Single-cell RNA sequencing analysis of mouse cardiac tissues with myocardial infarction

Project description:To investigate the transcriotome alteration in myocardial infarction (MI)-associated cells, we performed RNA sequcening analysis with single cells derived from mouse infarcted cardiac tissues or normal left ventricle (LV). Particularly, cardiac endothelial cells (ECs) were traced using a Cdh5-Cre;LSL-tdTomatom system.

2022-03-01 | GSE163956 | GEO

Total stranded RNA sequencing on bulk tumor or FACS isolated tumor cells from known rhabdomyosarcoma models

Project description:Comparision of aP2-Cre, Myog-Cre and Cdh5-Cre SmoM2 driven tumors shows that Cdh5-Cre tumors express muscle markers but have unique gene expression profiles; Comparision of aP2-Cre SmoM2/tdTomato tumor cells and Cdh5-Cre SmoM2/tdTomato tumor cells isolated by FACS for CD31-/tdTomato+ cells reveals that Cdh5-Cre tumors are not rhabdomyosarcoma

2025-09-22 | GSE283870 | GEO

Low-input unstranded RNA sequencing on bulk tumor or FACS isolated tumor cells from known rhabdomyosarcoma models [Low-input_BulkRNAseq]

Project description:Comparision of aP2-Cre, Myog-Cre and Cdh5-Cre SmoM2 driven tumors shows that Cdh5-Cre tumors express muscle markers but have unique gene expression profiles Comparision of aP2-Cre SmoM2/tdTomato tumor cells and Cdh5-Cre SmoM2/tdTomato tumor cells isolated by FACS for CD31-/tdTomato+ cells reveals that Cdh5-Cre tumors are not rhabdomyosarcoma

2025-09-22 | GSE231917 | GEO

Total stranded RNA sequencing on bulk tumor or FACS isolated tumor cells from known rhabdomyosarcoma models [Total-Stranded_RNAseq]

Project description:Comparision of aP2-Cre, Myog-Cre and Cdh5-Cre SmoM2 driven tumors shows that Cdh5-Cre tumors express muscle markers but have unique gene expression profiles Comparision of aP2-Cre SmoM2/tdTomato tumor cells and Cdh5-Cre SmoM2/tdTomato tumor cells isolated by FACS for CD31-/tdTomato+ cells reveals that Cdh5-Cre tumors are not rhabdomyosarcoma

2025-09-22 | GSE231916 | GEO

Smartseq3 Single-cell RNA-seq analysis of acutely FACS-purified tdTomato+ astrocyte derived cells from aged injured mice in vivo

Project description:To investigate the mechanisms that underlie astrocyte dedifferentiation, we performed single cell RNA sequencing analysis of tdTomato+ astrocyte derived cells approx 1 year after stab-wound injury. 2-3 month old GFAP-CreERT2;p53flox/flox;LSL-tdTomato (p53Gfap-icKO) mice were subjected to intracortical endoxifen injection to enable deletion of p53 gene and simultaneous expression of tdTomato for fate-mapping specifically in astrocytes (GFAP+) in the context of a stab-wound injury. GFAP-CreERT2;p53wt/wt;LSL-tdTomato (p53Gfapwt/wt) mice were used as control whereby p53 remains fate-mapped tdTomato+ astrocytes remain p53 wildtype after endoxifen injection. Approx 10-12 months after injury, mice were sacrificed and the peri-wound region was dissected. Mice of the same genotypes were pooled (3 p53Gfap-icKO and 2 p53Gfapwt/wt) and digested using the Miltenyi Adult brain dissociation kit. Single cell suspension was FACS sorted for tdTomato+ cells into 384 well plates and then Smartseq3 libraries were generated.

2023-02-23 | GSE220479 | GEO

Low-input unstranded RNA sequencing on FACS isolated tdTomato positive cells from the sternocleidomastoid muscles of aP2-Cre;R26tdTomato and Cdh5-Cre;R26tdTomato animals

Project description:Gene expression of tdTomato positive FACS isolated sternocleidomastoid msucles from aP2-Cre;R26tdTomato and Cdh5-Cre;R26tdTomato animals for ssGSEA analysis

2025-09-22 | GSE302641 | GEO

single cell gene expression analysis of aP2-Cre and Cdh5-Cre tdTomato labeled cells from multiple muscle [scRNA-seq]

Project description:Comparison of aP2-Cre and Cdh5-Cre tdTomato labaled cells from hindlimb and SCM muscles shows they mark the same endothelial cell populations

2025-09-22 | GSE231918 | GEO

Single-cell RNA-seq analysis of p53 knockout primary adult mouse astrocytes in vitro.

Project description:To investigate the mechansims that underlie astrocyte dedifferentiation, we performed single cell RNA sequencing analysis of primary astrocytes isolated from adult mouse cortex after p53 loss and exposure to mitogens, EGF and and FGF. Primary astrocytes were isolated from cortices of 2-3month old inducible p53 knockout mice (GFAP-CreERT2;p53flox/flox;LSL-tdTomato) using Miltenyi Adult Brain Dissociation kit and ACSA-2 beads. Treatment with 4-hydroxytamoxifen (4OHT) induces p53 loss and tdTomato labelling in GFAP+ astrocytes. Astrocytes were treated with 4OHT in media supplemented with EGF and FGF to induce recombination and astrocyte dedifferentiation in vitro.

2023-02-23 | GSE220477 | GEO

GBM microglia

Project description:High grade glioma in mice and human have been shown to be greatly impacted by the infiltration of macrophages in the tumor, but it is unknown whether the populations of macrophages in the tumors may have different roles in the tumor. Flow sorting and high throughput RNA-seq was employed to examine the two main populations of macrophages in the high grade mouse glioma, denoted by the high or low surface expression of CD45, and they were compared to the resident population of microglia in normal brain tissues that express low levels of CD45.

2019-02-27 | GSE65868 | GEO

Genome-wide DNA methylation profiling of 68 GBM (primary & recurrent), 4 glioma spheres and 5 non-tumor brain samples

Project description:DNA methylation analysis of 68 glioblastoma specimen of patients treated within clinical trials, 5 samples of normal brain tissue (non-tumor brain) and 4 tumor-derived glioma sphere lines. The data was used to identify changes in DNA methylation which contribute to the aberrant of expression of HOX transcription factors. Our group had previously demonstrated that expression of HOX genes was associated with increased resistance to chemo-radiotherapy and worse outcome in GBM patients Keywords: Disease state comparison

2015-01-06 | GSE60274 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data