Transcriptomics

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Using Zinc-based probes to generate pseudoislets from purified human alpha-cells for functional analyses


ABSTRACT: Investigation of the function of human alpha-cells remains a challenge due to the lack of simple and cost-effective purification methods to isolate high-quality alpha-cells from islets. Here, we introduce a novel and simple method for enriching live alpha-cells from dissociated human islet cells by using the reaction-based probe diacetylated Zinpyr1 (DA-ZP1) with > 97% purity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE199412 | GEO | 2023/09/29

REPOSITORIES: GEO

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