Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells

ABSTRACT: To explore the potential mechanism of chidamide in BM-MSC-mediated adipogenesis, we have employed mRNA microarray expression profiling platform to identify significant differential genes associated with adipogenic differentiation and chidamide management.

ORGANISM(S): Homo sapiens

PROVIDER: GSE199466 | GEO | 2022/04/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells

Project description:Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells

| PRJNA820040 | ENA

Expression profiling of mouse bone marrow skeletal progenitors with osteogenic and adipogenic potential and adipogenic progenitors treated with IFNγ.

Project description:Bone marrow stromal cells (BMSCs) were isolated from the femora and tibiae of irtTA-GBD*-TAg transgenic mice. Using cellular cloning we established skeletal progenitors with unipotent osteogenic and adipogenic properties. Previous RNA-seq analysis of more progenitor types revealed differential expression in members of the Interferon-gamma (IFNγ) signaling pathway. Treatment of adipogenic progenitors with IFNγ inhibited adipogenesis and promoted osteogenesis. RNA-seq analysis of osteogenic, adipogenic and IFNγ treated adipogenic clones revealed factors controlling the osteogenic versus adipogenic commitment of bone marrow skeletal progenitors.

2018-07-02 | GSE114475 | GEO

Human BMSCs protein analysis that interact with TRAF4 during adipogenic differentiation

Project description:To explore the mechanism underlying the negative regulation of adipogenesis by TRAF4, we performed the LC-MS/MS experiments to identify the proteins that interact with TRAF4 during adipogenic differentiation.

2020-05-27 | PXD017524 | Pride

Brown Adipogenic Reprogramming Induced by a Small Molecule

Project description:Transcriptome analysis of early adipogenesis induced by basal adipogenesis medium(AM) and AM+Bex for 2 days in C2C12 cells Global gene expression profiling has shown Bex induced adipogenic genes expression change We analyzed 4 samples from basal adipogenesis medium(AM) and AM+Bex(10uM) treated C2C12 cells using the Affymetrix Mouse Gene 1.0 ST platform. Array data was processed by Affymetrix Expression Console and Transcriptome Analysis Console (TAC) software.

2013-11-15 | E-GEOD-52364 | biostudies-arrayexpress

Brown Adipogenic Reprogramming Induced by a Small Molecule

2013-11-15 | GSE52364 | GEO

MicroRNA array analysis of mature microRNAs of adipogenic differentiated human bone marrow stromal cells

Project description:In bone marrow, mesenchymal stromal cells (BMSCs), the precursors of adipocytes and osteoblasts, are exposed to a plethora of stimuli that determine the balance between adipogenesis and osteogenesis which in turn are competing and reciprocal. The differentiation of BMSCs, in fact, is a two-step process, lineage commitment (from MSCs to lineage-specific pro-genitors) and maturation (from progenitors to specific cell types). Adipogenesis is a finely tuned multi-step process requiring the sequential activation of numerous transcription factors driving the typical physiological and morphological changes observed in the progenitor cells, i.e., cell cycle arrest, metabolic reprogramming, and lipid accumulation. Among the small non-coding RNAs, microRNAs represent an additional mechanism for controlling adipogenic gene expression. Given their unique ability to simultaneously regulate multiple protein targets and processes, it has been suggested that microRNAs may play a leading role in BMSC differentiation. Here, we evaluated miRNAs differentially regulated during human BMSC adipogenic differentiation in vitro.

2021-12-01 | GSE189508 | GEO

Development of a 3D fatty infiltration platform to model, at higher scale, the impact of LY2090314 compound on fibro/adipogenic progenitor adipogenic drift

Project description:In human dystrophies, the progressive muscle wasting is exacerbated by ectopic deposition of fat and fibrous tissue originating from fibro/adipogenic progenitors (FAPs). In degenerating muscles, the ability of these cells to adjuvate a successful healing is attenuated and FAPs aberrantly expand and differentiate into adipocytes and fibroblasts. Thus, arresting the fibroadipogenic fate of FAPs, without affecting their physiological role, represents a valuable therapeutic strategy for patients affected by muscle diseases. Here, using a panel of adipose progenitor cells including human-derived FAPs coupled with pharmacological perturbations and proteome profiling, we report that LY2090314 interferes with a genuine adipogenic program acting as WNT surrogate for the stabilization of a competent -catenin transcriptional complex. To predict the beneficial impact of LY2090314 in limiting ectopic deposition of fat in human muscles, we combined the Poly-Ethylene-Glycol-Fibrinogen biomimetic matrix with these progenitor cells to create a miniaturized 3D model of adipogenesis. Using this scalable system, we demonstrated that a two-digit nanomolar dose of this compound is effective to repress adipogenesis in a higher 3D scale, thus offering a concrete proof for the use of LY2090314 to limit FAP-derived fat infiltrates in dystrophic muscles.

2023-06-13 | PXD040479 | Pride

KD of Fibroblast specific TF

Project description:Combinatorial knock down of LHX9, OSR1, PRRX1, TWIST2, fibroblast specific transcription factors and adipogenic induction by medium change induce transdifferentiation from fibroblast to adipocyte. Adipogenesis from meshencymal stem cell (MSC) and human preadipocyte (PreADP) were used for general reference of adipogenesis.

2013-11-12 | GSE44303 | GEO

Secretome analysis of human bone marrow derived mesenchymal stromal cells

Project description:As an essential cellular component of the bone marrow (BM) microenvironment mesenchymal stromal cells (MSC) play a pivotal role for the physiological regulation of hematopoiesis, in particular through the secretion of cytokines and chemokines. Mass spectrometry (MS) facilitates the identification and quantification of a large amount of secreted proteins (secretome), but can be hampered by the false-positive identification of contaminating proteins released from dead cells or derived from cell medium. To reduce the likelihood of contaminations we applied an approach combining secretome and proteome analysis to characterize the physiological secretome of BM derived human MSC. Our analysis revealed a secretome consisting of 315 proteins. Pathway analyses of these proteins revealed a high abundance of proteins related to cell growth and/or maintenance, signal transduction and cell communication thereby representing key biological functions of BM derived MSC on protein level. Within the MSC secretome we identified several cytokines and growth factors such as VEGFC, TGF-β1, TGF-β2 and GDF6 which are known to be involved in the physiological regulation of hematopoiesis. By comparing the peptide patterns of secretomes and cell lysates 17 proteins were identified as candidates for proteolytic processing. Taken together, our combined MS work-flow reduced the likelihood of contaminations and enabled us to carve out a specific overview about the composition of the secretome from human BM derived MSC. This methodological approach and the specific secretome signature of BM derived MSC may serve as basis foffuture comparative analyses of the interplay of MSC and HSPC in patients with hematological malignancies.

2019-02-12 | PXD011643 | Pride

KD of Fibroblast specific TF

2013-11-12 | E-GEOD-44303 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data