CD300ld regulates tumor immunity - In vivo Crispr-Cas9 screening

ABSTRACT: To identify surface proteins on myeloid cells involved in the establishment of the tumor immune microenvironment, we selected transmembrane protein encoding genes with a high myeloid/lymphoid expression ratio to construct a sgRNA library. The sgRNA pool was transduced into hematopoietic stem/progenitor cells of Cas9 mice to transplant the lethal irradiated mice for immune system reconstitution, then followed by B16-F10 tumor inoculation. Once the tumor was established, the tumor and bone marrow (BM) were collected for sgRNA deep sequencing and comparison.

ORGANISM(S): Mus musculus

PROVIDER: GSE199603 | GEO | 2023/06/26

REPOSITORIES: GEO