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The nuclear granule Paraspeckles interacts with the chromatin remodeling SWI/SNF complex to regulate alternative splicing

ABSTRACT: There are three major goals of the study 1) Understand if the differential effect of depletion of ARID1 paralogs on the trancriptome of HEK293FRT cells 2) Understand if NEAT1 depletion effects any alternative splicing pathways in HEK293FRT cells 3) Is the regulation of alternative splicing pathways by NEAT1 ARID1B dependent?

ORGANISM(S): Homo sapiens

PROVIDER: GSE199631 | GEO | 2022/11/10

REPOSITORIES: GEO

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Expression data from triple negative breast cancer cells

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Knockout mice reveal an essential role for Epithelial splicing regulatory proteins (Esrps) in mammalian development and epithelial splicing in vivo

Project description:Tissue- and cell-type specific regulators of alternative splicing (AS) are an essential layer of posttranscriptional gene regulation necessary for normal cellular function, patterning, and development. Here we report the Epithelial splicing regulatory proteins (Esrps) are required for patterning of multiple organs, with loss of both paralogs, Esrp1 and Esrp2, resulting in increasingly severe phenotypes. Global profiling of the Esrp splicing regulatory network from total epidermis revealed varied splicing sensitivity of Esrp targets upon loss of Esrp1 or double knockout. This may explain the progressive phenotypes seen in Esrp knockout mice, and these mice provide a unique genetic tool to evaluate functional consequences of epithelial splicing events in vivo.

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Transcriptome analysis of alternative splicing under ABA exposure in osfkbp20-1b mutant compared to wild-type (ZH11) of rice

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Transriptome analysis of alternative splicing under heat stress condition in atcyp18-2 mutant compared to Wild type (Col-0) of Arabidopsis

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An EMT-driven alternative splicing program occurs in human breast cancer and modulates cellular phenotype.

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