Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Protracted neuronal recruitment in the temporal lobe of young children

ABSTRACT: The human brain's temporal lobe contains the entorhinal cortex (EC), a highly interconnected integrative hub for sensory and spatial information; key for episodic memory formation and the primary source of cortical hippocampal inputs. The human EC continues to develop during childhood, yet neurogenesis and neuronal migration to the EC are widely considered complete by birth. Here we show that the human temporal lobe contains many young neurons migrating into the postnatal EC and adjacent regions; with a large tangential stream persisting ~1 year and radial dispersal until ~2-3 years. In contrast, we found no equivalent postnatal migration in rhesus macaque (Macaca mulatta). Immunostaining and single-nuclei RNA-seq (snRNA-seq) of ganglionic eminence (GE) germinal zones, EC stream, and postnatal EC reveals that most EC stream migrating cells are derived from the caudal GE and become LAMP5+RELN+ inhibitory interneurons. These late-arriving interneurons could continue to shape the processing of sensory and spatial information well into postnatal life when children are actively interacting with their environment. The EC is one of the first brain regions affected in Alzheimer’s disease and recent work links the decline in cognitive function to the loss of LAMP5+RELN+ cells. Our investigation reveals that many of these cells arrive in the EC through a major postnatal migratory stream in early childhood.

ORGANISM(S): Homo sapiens

PROVIDER: GSE199762 | GEO | 2023/09/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Adrenomedullin promotes the migration of interneurons in a human forebrain assembloid model for hypoxic interneuronopathy of prematurity.

Project description:We developed a model for hypoxic injury of migrating human cortical interneurons using forebrain organoids and forebrain assembloids derived from hiPSCs.

2023-09-01 | GSE183201 | GEO

Protein profile of vitreous from Reeler model

Project description:To provide information about candidate factors contributing to impaired retinogenesis that might be useful tool for alternative therapeutic strategies, proinflammatory/profibrogenic mediator were evaluated in the vitreous from Reelin deficient mice, a model characterized by impaired retinogenesis. Vitreous samples from Reeler (n=9; RELN-/-) and WT (n=9; wild-type RELN+/+) mice at different postnatal (14, 21, 28) days, were used. Some potential candidate proteins were analyzed by chip array and confirmed by conventional analysis.

2019-02-13 | E-MTAB-7622 | biostudies-arrayexpress

ScRNAseq - Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration

Project description:We use cerebral organoid fusions and a novel migration tracking platform to understand the functional impact of neurotransmitter signaling on migrating human interneurons.

2021-10-20 | GSE161550 | GEO

BulkRNAseq - Neurotransmitters regulate distinct phases of multimodal human interneuron migration

Project description:We use cerebral organoid fusions and a novel migration tracking platform to understand the functional impact of neurotransmitter signaling on migrating human interneurons.

2021-10-20 | GSE182614 | GEO

Expression profiling in cortical GABAergic neurons at neonate

Project description:During embryonic development, GABAergic interneurons, a main inhibitory component in the cerebral cortex, migrate tangentially from the ganglionic eminence (GE) to cerebral cortex. After reaching the cerebral cortex, they start to extend their neurites for constructing local neuronal circuits around the neonatal stage. Aberrations in migration or neurite outgrowth are implicated in neurological and psychiatric disorders such as epilepsy, schizophrenia and autism. Previous studies revealed that in the early phase of cortical development the neural population migrates tangentially from the GE in the telencephalon and several genes have been characterized as regulators of migration and specification of GABAergic interneurons. However, much less is known about the molecular mechanisms of GABAergic interneurons-specific maturation at later stages of development. Here, we performed genome-wide screening to identify genes related to the later stage by flow cytometry based-microarray (FACS-array) and identified 247 genes expressed in cortical GABAergic interneurons. Among them, Dgkg, a member of diacylglycerol kinase family, was further analyzed. Correlational analysis revealed that Dgkg is dominantly expressed in somatostatin (SST)-expressing GABAergic interneurons. The functional study of Dgkg using GE neurons indicated alteration in neurite outgrowth of GABAergic neurons. This study shows a new functional role for Dgkg in GABAergic interneurons as well as the identification of other candidate genes for their maturation.

2017-12-01 | GSE93295 | GEO

Transcriptional maintenance of cortical somatostatin interneuron subtype identity during migration

Project description:Cortical interneuron diversity arises from the interplay of intrinsic developmental patterning and local extrinsic cues. While individual genetic programs underlying cardinal cell type identity are established in immature neurons prior to integration into cortical circuits, it remains unclear whether distinct interneuron subtype identities are pre-established, and if so, how their identity is maintained prior to circuit integration. Sox6 is a transcription factor with an established role in the maturation of interneurons derived from the medial ganglionic eminence and cell-type specification in other neuronal and non-neuronal cells. To determine a possible role in maintaining cortical somatostatin-expressing (Sst+) interneuron subtype identity, we conditionally removed Sox6 (Sox6-cKO) in migrating Sst+ interneurons and assessed the effects on their mature identity. In adolescent animals, five of eight molecular Sst+ subtypes were nearly absent in the cortex of Sox6-cKO mice. This reduced subtype diversity was not due to a decrease in the overall number of Sst+ interneurons and cells displayed electrophysiological maturity and expressed genes enriched within the broad class of Sst+ interneurons. Furthermore, we show that at embryonic day 18.5, prior to cortical integration, mature Sst+ cell subtype identity could already be inferred in both control and Sox6-cKO cortices, suggesting that the loss in subtype diversity observed in the mature cortex is due to a disrupted subtype maintenance. Importantly, Sox6 removal at postnatal day 7, after Sst+ interneurons have finished migrating and begun integration into the network, did not disrupt marker expression of Sst+ subtypes in the mature cortex. Therefore, Sox6 is necessary during this migratory phase for maintenance of Sst+ subtypes identity, indicating that subtype maintenance requires active transcriptional programs during migration.

2023-08-25 | GSE233791 | GEO

Disruption of KCC2 in parvalbumin-positive interneurons causes fatal epilepsy in mice

Project description:Since many years, we are interested in the regulation of the intraneuronal chloride concentration. We were the first group reporting a full knockout of the KCl-co-transporter KCC2, which dies immediately after birth due to respiratory failure. Notably, KCC2 loss-of-function mutations are associated with inherited febrile seizures, severe genetic generalized epilepsy and epilepsy of infancy with migrating focal seizures. Here, we used our floxed line to study the consequences of the disruption of KCC2 within parvalbumin-positive interneurons in mice. Remarkably, this leads to the disinhibition of PV-positive interneurons as evidenced by a decrease of E-S-Coupling and an increase in the sIPSC frequency. Nevertheless, these mice develop fatal epilepsy with progressive loss of parvalbumin-positive interneurons thus increasing network excitability.

2022-11-02 | E-MTAB-11147 | biostudies-arrayexpress

MTG8 interacts with LHX6 to specify cortical interneuron subtype identity

Project description:In the study we compared migrating embryonic cortical interneurons from control mouse embryos and ones carryng homozygous deletions of either Mtg8 or Lhx6. The aim was to identify genes that are co-regulated by LHX6 and MTG8.

2022-08-21 | GSE195743 | GEO

Comparative expression of embryonic medial- and lateral/caudal-ganglionic eminence cells migrating in the mouse cerebral cortex

Project description:Cortical interneurons originate in the medial and caudal ganglionic eminence and migrate into the cortex during embryogenesis. We purified cells migrating within the cortex at different embryonic stages and compared their transcriptome to identify transcriptional programmes underlying distinct cortical interneuron fates.

2022-08-21 | GSE207506 | GEO

LAMP-5 is an essential inflammatory-signaling regulator and novel immunotherapy target for MLL-rearranged acute leukemia

Project description:In this study, we demonstrate that LAMP5 is essential for proliferation of MLL-rearranged leukemias. Through gene expression changes occurring upon LAMP5 knockdown, we show that LAMP5 maintains an important balance between Type I interferon and NFkB signaling, and that knockdown biases MLL leukemia cells towards activation of the interferon pathway

2022-08-07 | GSE166523 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data