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Gene expression profiles of P7 bladder epithelium compartments isolated from Upk1b mice. (GUDMAP Series ID: 34)

ABSTRACT: The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing genitourinary tract The central thesis is straightforward. The combination of microdissected tissues and FACS sorted cells plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here delineates the gene expression profiles of the epithelial compartments of the P7 mouse bladder.

ORGANISM(S): Mus musculus

PROVIDER: GSE19979 | GEO | 2010/01/22

SECONDARY ACCESSION(S): PRJNA120133

REPOSITORIES: GEO

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Transcription profiling of mouse P7 bladder epithelium compartments: uroplakin expressing (+ve) vs. non-expressing (-ve) epithelial cells (GUDMAP Series_id: 34)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing genitourinary tract The central thesis is straightforward. The combination of microdissected tissues and FACS sorted cells plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here delineates the gene expression profiles of the epithelial compartments of the P7 mouse bladder. Experiment Overall Design: At postnatal day 7 mice were euthanized by CO2 asphyxiation and the bladders were removed and cut at the bladder neck. The bladder was cut into rings and treated with 1 mg/ml trypsin in TyrodeÃs solution for 30 minutes at 37 degrees C. The layers were separated by gentle microdissection and the epithelial layer was further digested in a mixture of Blendzymes1/4 at 37 degrees C until a single cell suspension was obtained The samples were placed in RLT and stored at -80 degrees C.RNA was prepared and the Epicenter 2-round amplification scheme described on the web was performed. The Affymetrix GeneChip Mouse Genome 430 2.0 Array was used to interrogate the amplified RNA.

2010-02-17 | E-GEOD-19979 | biostudies-arrayexpress

Gene expression profiles of E15 ureter. (GUDMAP Series ID: 26)

2009-04-25 | GSE15795 | GEO

Gene expression profiles of E15 ureter (GUDMAP Series ID: 26)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing genitourinary tract. The central thesis is straightforward. The combination of microdissected tissues and FACS sorted cells plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing urogenital system. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. The data submitted here delineates the gene expression profiles of the e15 mouse ureter. Comparisons with the same compartments in the neonate will be made to determine changes in gene expression from relatively undifferentiated tissue (e15) to a highly differentiated structure (neonate). FVB/N mice were time mated. At embryonic day 15 mice were euthanized by decapitation and the ureters were microdissected, cut from pelvis to the bladder insertion point and snap frozen in liquid nitrogen. Total RNA was isolated for gene expression analysis using the Affymetrix GeneChip Mouse Genome 430 2.0 Array.

2009-04-25 | E-GEOD-15795 | biostudies-arrayexpress

Gene expression profiles of adult renal corpusle isolated using sieving techniques. (GUDMAP Series ID: 30)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The use of microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone.

2010-07-16 | GSE17141 | GEO

Gene expression profiles of adult renal capsule isolated from kidneys using micro-dissection. (GUDMAP Series ID: 36)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone.

2010-03-06 | GSE20639 | GEO

Transcription profiling of mouse developing kidney (GUDMAP Series_id: 21)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. Experiment Overall Design: At specific developmental time points we isolate discrete elements of the kidney by using fluorescent activated cell sorting (FACS) and then define their gene expression profiles with microarrays.

2008-10-20 | E-GEOD-11232 | biostudies-arrayexpress

Transcription profiling of mouse developing kidney (GUDMAP Series_id: 12)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in laser capture microdissected components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. Experiment Overall Design: At different developmental time points we isolate discrete elements of the kidney by using laser capture microdissection and then define their gene expression profiles with microarrays.

2008-06-15 | E-GEOD-6589 | biostudies-arrayexpress

Transcription profiling of mouse developing kidney (GUDMAP Series_id: 17)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. Experiment Overall Design: At different developmental time points we isolate discrete elements of the kidney by using fluorescent activated cell sorting (FACS) and then define their gene expression profiles with microarrays.

2008-06-16 | E-GEOD-8232 | biostudies-arrayexpress

Transcription profiling of mouse developing kidney (GUDMAP Series_id: 18)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of laser capture microdissection (LCM) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in laser capture microdissected components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. Experiment Overall Design: At different developmental time points we isolate discrete elements of the kidney by using laser capture microdissection and then define their gene expression profiles with microarrays.

2008-06-16 | E-GEOD-8360 | biostudies-arrayexpress

Gene expression profiles of E15.5 endothelial cells isolated from TIE2-GFP transgenic mice using FACS. (GUDMAP Series ID:38)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone.

2010-03-23 | GSE20991 | GEO

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