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Adaptation of group A Streptococcus to human amniotic fluid

ABSTRACT: Background. For more than 100 years, group A Streptococcus has been identified as a cause of severe, and in many cases fatal, infections of the female urogenital tract. Due to advances in hospital hygiene and the advent of antibiotics, this type of infection has been virtually eradicated. However, within the last three decades there has been an increase in severe intra- and post-partum infections attributed to GAS. Principal Findings. The majority of changes in the GAS transcriptome involved down regulation of multiple adhesins and virulence factors and activation of the stress response. We observed significant changes in genes involved in the arginine deiminase pathway and in the nucleotide de novo synthesis pathway. Conclusions/Significance. Our work provides new insight into how pathogenic bacteria respond to their environment to establish infection and cause disease.

ORGANISM(S): Streptococcus pyogenes MGAS6180

PROVIDER: GSE19985 | GEO | 2010/03/15

SECONDARY ACCESSION(S): PRJNA120145

REPOSITORIES: GEO

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Adaptation of group A Streptococcus to human amniotic fluid

Project description:Background. For more than 100 years, group A Streptococcus has been identified as a cause of severe, and in many cases fatal, infections of the female urogenital tract. Due to advances in hospital hygiene and the advent of antibiotics, this type of infection has been virtually eradicated. However, within the last three decades there has been an increase in severe intra- and post-partum infections attributed to GAS. Principal Findings. The majority of changes in the GAS transcriptome involved down regulation of multiple adhesins and virulence factors and activation of the stress response. We observed significant changes in genes involved in the arginine deiminase pathway and in the nucleotide de novo synthesis pathway. Conclusions/Significance. Our work provides new insight into how pathogenic bacteria respond to their environment to establish infection and cause disease. To identify genes that were up or down regulated in response to growth in AF, GAS was grown in human AF or standard laboratory media (THY) and samples for expression microarray analysis were collected during mid-logarithmic, late-logarithmic, and stationary growth phases. Microarray analysis was performed using a custom Affymetrix chip and normalized hybridization values derived from three biological replicates were collected at each growth point. Ratios of AF/THY above a 2-fold change and P-value < 0.05 were considered significant.

2010-05-05 | E-GEOD-19985 | biostudies-arrayexpress

Comparative Genome Analysis of Ureaplasma parvum Clinical Isolates

Project description:Ureaplasma are widespread parasites colonizing the mucosal surface of the human urogenital tract, and it has been suspected as a causative agent of nongonococcal urethritis, pregnancy complications and prenatal infections. Ureaplasma may also cause central nervous system infections and affect the lower respiratory tract of newborn babies. However, Ureaplasma spp. have also been detected in the urogenital tracts of clinically healthy patients, and their role in the development of infections thus remains unclear. Like in other organisms, virulence of Ureaplasma is determined by the presence of virulence factors - adhesions, human IgA protease, phospholipase and urease. However, the existence of interrelationships between the presence of these genes in the Ureaplasma genome and the incidence of diseases in man has not been demonstrated. Difficulties in the elucidation of these interrelationships may arise from significant macro- (gene mutation, chromosomal rearrangements) and micro- (nucleotide polymorphism) genomic heterogeneity. It is possible that the combination of the variable strain-specific genes in Ureaplasma with generally known virulence factors determine the development of pathological processes on the mucosal surface of the human urogenital tract. In our research we used 10 clinical and 1 laboratory strain

2010-07-01 | E-GEOD-5378 | biostudies-arrayexpress

Comparative Genome Analysis of Ureaplasma parvum Clinical Isolates

Project description:Ureaplasma are widespread parasites colonizing the mucosal surface of the human urogenital tract, and it has been suspected as a causative agent of nongonococcal urethritis, pregnancy complications and prenatal infections. Ureaplasma may also cause central nervous system infections and affect the lower respiratory tract of newborn babies. However, Ureaplasma spp. have also been detected in the urogenital tracts of clinically healthy patients, and their role in the development of infections thus remains unclear. Like in other organisms, virulence of Ureaplasma is determined by the presence of virulence factors - adhesions, human IgA protease, phospholipase and urease. However, the existence of interrelationships between the presence of these genes in the Ureaplasma genome and the incidence of diseases in man has not been demonstrated. Difficulties in the elucidation of these interrelationships may arise from significant macro- (gene mutation, chromosomal rearrangements) and micro- (nucleotide polymorphism) genomic heterogeneity. It is possible that the combination of the variable strain-specific genes in Ureaplasma with generally known virulence factors determine the development of pathological processes on the mucosal surface of the human urogenital tract. Keywords: macroarray, variability of genome, Ureaplasma parvum

2006-07-25 | GSE5378 | GEO

The peroxiredoxin Asp f3 acts as redox sensor in Aspergillus fumigatus

Project description:The human pathogenic fungus Aspergillus fumigatus is readily eradicated by the innate immunity of immunocompetent human hosts, but can cause severe infections, such as invasive aspergillosis (IA), in immunocompromised individuals. During infection, the fungal redox homeostasis can be challenged by reactive oxygen (ROS) species, either derived from the oxidative burst of innate immune cells or the action of antifungal drugs. The peroxiredoxin Asp f3 was found to be essential to cause IA in mice, but how Asp f3 integrates to fungal redox homeostasis remains unknown. Here, we show that in vivo, Asp f3 acts as a sensor for ROS. While global transcription in fungal hyphae under minimal growth conditions was fully independent of Asp f3, a robust induction of the oxidative stress response required the presence of the peroxiredoxin. Hyphae devoid of Aspf 3 failed to activate several redox active genes, like members of the gliotoxin biosynthesis gene cluster and integral members of the Afyap1 regulon, the central activator of the ROS defence machinery in fungi. Upon deletion of the asp f3 gene Afyap1 displayed significantly reduced nuclear localization during ROS exposure, indicating that Asp f3 can act as an intracellular redox sensor for several target proteins

2021-06-03 | GSE173349 | GEO

Actinotignum schaalii

Project description:Can cause urinary tract infections

| PRJNA52215 | ENA

miRNA expression data from post-partum dairy cattle (Holstein Friesian) liver

Project description:High yielding dairy cattle undergo a state of NEB (negative energy balance) during the post-partum period when energy demand for lactation and maintenance exceeds energy intake. During this period in order to counteract NEB the liver under goes extensive metabolic and physiological change resulting in alteration in hepatic genes and miRNAs expression. We used Affymetrix Multispecies miRNA-2_0 Array with miRBase version 15 coverage to assess the liver miRNA expression in SNEB (severe NEB) and MNEB (mild NEB) Holstein Friesian cattle during the post-partum period. A NEB model of Holstein Friesian was established such that 12 post-partum cattle were randomly assigned to MNEB and SNEB groups depending on different feeding and milking regimes

2013-10-25 | E-GEOD-51658 | biostudies-arrayexpress

miRNA expression data from post-partum dairy cattle (Holstein Friesian) liver

2013-10-25 | GSE51658 | GEO

streptococcus suis LC-MS/MS - MnmE, a Central tRNA-Modifying GTPase, Is Essential for the Growth, Pathogenicity, and Arginine Metabolism of Streptococcus suis Serotype 2

Project description:Streptococcus suis is an important pathogen in pigs and can also cause severe infections in humans. However, little is known about proteins associated with cell growth and virulence of S. suis. In this study, a GTPase MnmE homologue was identified in a Chinese isolate (SC19) that drives a tRNA modification reaction. A mnmE deletion strain (ΔmnmE) and a complementary strain (CΔmnmE) were constructed to systematically decode the characteristics and the function of MnmE by applying in vitro and in vivo studies, and proteomic analysis. Phenotypic analysis revealed that the ΔmnmE strain displayed deficient growth, attenuated pathogenicity, perturbed arginine metabolic pathway mediated by arginine deiminase system (ADS). Consistently, TMT-based quantitative proteomics analysis confirmed that 491 proteins were differentially expressed (238 up- and 253 down-regulated) between strains ΔmnmE and SC19. Many proteins associated with DNA replication, cell division, and virulence were down-regulated. Particularly, the core enzymes of ADS were significantly down-regulated in strain ΔmnmE. These data also provided putative molecular mechanisms for MnmE in cell growth and survival in an acidic environment. Therefore, MnmE is a central regulator that plays an important role in cell growth and virulence, as well as arginine metabolism.

2019-11-13 | PXD012716 | Pride

Homo sapiens

Project description:INTS8 mutations cause severe neurodevelopmental syndrome

| PRJNA308838 | ENA

Global transcriptome analysis of colonic tissue during Clostridioides difficile infection

Project description:Clostridioides difficile can cause severe infections in the gastrointestinal tract and affects almost half a million people in the U.S every year. Upon establishment of infection, a strong immune response is induced. We sought to investigate the dynamics of the mucosal host response during C. difficile infection.

2023-09-13 | GSE237574 | GEO

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