Genomics

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Minicircle-derived iPS cells - Agilent Data


ABSTRACT: Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem (iPS) cells. We report the use of ‘minicircle’ DNA, a vector type that is free of bacterial DNA and capable of high expression in cells. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells. (Note: Our Nature Methods publication included analysis of array data from GSM374067 and GSM374068 in conjunction with this series).

ORGANISM(S): Homo sapiens

PROVIDER: GSE20014 | GEO | 2010/02/01

SECONDARY ACCESSION(S): PRJNA124087

REPOSITORIES: GEO

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