Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing Analysis of control and siSRSF2 treated RKO cells Transcriptomes

ABSTRACT: We treated the RKO cells with siSRSF2 or control siRNA for 48 hours.Then, we extracted RNAs and performed next generation sequencing. By comparing sequencing data from control and siSRSF2 samples, we profiled the alternative splicing events and gene expression regulated by SRSF2 in CRC.

ORGANISM(S): Homo sapiens

PROVIDER: GSE200272 | GEO | 2023/06/02

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Next Generation Sequencing Analysis of control and siSRSF1 treated RKO cell Transcriptomes

Project description:We treated the RKO cells with siSRSF1 or control siRNA for 48 hours. Then we extracted the RNAs and performed the next generation sequencing. By comparing sequcing data from siSRSF1 and contron siRNA treated samples, we profiled the gene expression regulated by siRNA.

2018-05-01 | GSE98508 | GEO

Next Generation Sequencing Analysis of control and hnRNPK-/- RKO cells Transcriptomes

Project description:We generated the hnRNPK-/- RKO cells,and collected the cells at 2 days after transfectd with siRNA. Then,we extracted RNAs and performed next generation sequencing. By comparing sequcing data from control and hnRNPK -/- samples, we profiled the alternative splicing events and gene expression regulated by hnRNPK in CRC.

2018-10-26 | GSE85522 | GEO

Next Generation Sequencing Analysis of control and siCHERP treated RKO cell Transcriptomes

Project description:We treated the RKO cells with siCHERP or control siRNA for 72 hours. Then we extracted the RNAs and performed the next generation sequencing. By comparing sequcing data from siCHERP and contron siRNA treated samples, we profiled the gene expression regulated by siRNA.

2019-06-01 | GSE115323 | GEO

Next Generation Sequencing Analysis of control and SRSF2-/- Huh7 cells Transcriptomes

Project description:We generated the SRSF2-/- Huh7 cells,and collected the cells at 2 days after transfected with siRNA. Then, we extracted RNAs and performed next generation sequencing. By comparing sequencing data from control and SRSF2 -/- samples, we profiled the alternative splicing events and gene expression regulated by SRSF2 in HCC.

2017-01-17 | GSE78705 | GEO

Next Generation Sequencing Analysis of control and knockdown of hnRNPU in RKO cells

Project description:We knockdown the control and the hnRNPU gene in the RKO cells, then extracted the total RNAs and performed the next generation sequencing. By comparing sequencing data from control and knockdown of hnRNPU cells,we profield the downstream targets of the hnRNPU gene.

2022-12-10 | GSE141771 | GEO

Gene regulation by restoration of KMT2C in human colorectal cancer cells HCT116 and RKO

Project description:The histone 3 lysine 4 (H3K4) monomethylase KMT2C is mutated across several cancer types, however the effects of mutations on epigenome organization, gene expression and cell growth are not clear. To study effects of KMT2C expression in CRC cells we restored one allele to wild type KMT2C in the two CRC cell lines RKO and HCT116, which both are homozygous KMT2C c.8390delA mutant, by gene editing. RNA sequencing was performed to profile gene expression when KMT2C was restored in RKO and HCT116 cells.

2020-06-01 | GSE131507 | GEO

Expression data of TAGLN-overexpressing RKO human colon cancer cells

2013-07-18 | GSE48998 | GEO

Next Generation Sequencing Analysis of control and siCHERP treated RKO cell Transcriptomes

Project description:Next Generation Sequencing Analysis of control and siCHERP treated RKO cell Transcriptomes

| PRJNA474532 | ENA

Expression data of TAGLN-overexpressing RKO human colon cancer cells

Project description:Transgelin was the top-ranked marker of metastatic potential identified in the comparison of node-positive colorectal cancer (CRC) versus node-negative CRC in our previous study. Transgelin is localized in the nucleus of cultured CRC cells and microRNA-mediated knockdown of TAGLN (the gene encoding transgelin) expression modulates the expression of genes involved in the epithelial-to-mesenchymal transition. We performed gene expression profiling on control and transgelin-overexpressing RKO cells using Affymetrix microarray technology. The plasmid carrying TAGLN was generated using the pcDNA6.2/EmGFP-Bsd/V5-DEST vector (Invitrogen, Carlsbad, CA) and the pDONR221-TAGLN-mut plasmid from a previous study by an LR recombination reaction (http://www.invitrogen.com). After lipofectamine-mediated transfection of the TAGLN-carrying plasmid or the vector-only control plasmid, stable transfectants were selected and cultured in medium containing 25 Î¼g/ml blasticidin followed by RNA extraction and hybridization on Affymetrix microarrays. Six samples were obtained from three independent experiments (technical replicates).

2013-07-18 | E-GEOD-48998 | biostudies-arrayexpress

Next Generation Sequencing Analysis of Wild Type and SRSF2-/- D11 mice liver Transcriptomes

Project description:We generated the liver specific SRSF2 -/- mice, and collected their liver at 11 day after birth and controls. Then, we extracted RNAs and performed next generation sequencing. By comparing sequencing data from WT and SRSF2 -/- samples, we profiled the alternative splicing events and gene expression regulated by SRSF2 during mouse liver development process.

2016-04-19 | GSE73536 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data