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Diurnal expression data from developing barley caryopses

ABSTRACT: Caryopses of barley (Hordeum vulgare), like all other cereal seeds, are complex sink organs optimized for storage starch accumulation and embryo development. Their development from early stages after pollination to late stages of seed ripening has been studied in great detail. However, information on the caryopses’ diurnal adaptation to changes in light, temperature and alterations in phloem-supplied carbon and nitrogen remained unknown. In this study, we applied the 22K Barley1 GeneChip microarray to investigate diurnal gene regulation events of barley caryopses at 11 to 12 days post anthesis.

ORGANISM(S): Hordeum vulgare

PROVIDER: GSE20034 | GEO | 2010/03/25

SECONDARY ACCESSION(S): PRJNA124123

REPOSITORIES: GEO

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Diurnal expression data from developing barley caryopses

Project description:Caryopses of barley (Hordeum vulgare), like all other cereal seeds, are complex sink organs optimized for storage starch accumulation and embryo development. Their development from early stages after pollination to late stages of seed ripening has been studied in great detail. However, information on the caryopsesâ?? diurnal adaptation to changes in light, temperature and alterations in phloem-supplied carbon and nitrogen remained unknown. In this study, we applied the 22K Barley1 GeneChip microarray to investigate diurnal gene regulation events of barley caryopses at 11 to 12 days post anthesis. Developing barley caryopses were harvested at different time-points of a day (at 7:00 AM, 2:00 PM, 5:00 PM and 8:00 PM) including dark samples (6:00 AM and 10:00 PM). Pooled caryopses (form 3 Plants) from the respective time points were used for RNA extraction and hybridization on Affymetrix microarrays (Barley1 GeneChip; GEO accsession GPL1340). Biological replicates were performed.

2010-04-07 | E-GEOD-20034 | biostudies-arrayexpress

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Heat stress expression data from developing barley caryopses

Project description:High temperature stress, like any abiotic stress, impairs the physiology and development of plants, including the stages of seed setting and ripening. In this study we used the 22K Barley1 GeneChip microarray to investigate the response of developing barley (Hordeum vulgare) caryopses at 12 days post anthesis to 0.5h, 3h and 6h of heat stress exposure. Developing barley caryopses were harvested at different time-points of a day during heat stress (42M-BM-0C) (2:00 PM, 5:00 PM and 8:00 PM). Heat stress started at 1:30PM. Pooled caryopses (form 3 plants) from the respective time points were used for RNA extraction and hybridization on Affymetrix microarrays (Barley1 GeneChip; GEO accsession GPL1340). Biological replicates were performed.

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