Methylated RNA immunoprecipitation sequencing in the Kasumi-1 cell line treated with CoCl2 and echinomycin

ABSTRACT: To evaluate the role of HIF1α in modulating the m6A abundance, we conducted m6A sequencing in the Kasumi-1 cell line treated with CoCl2 and echinomycin. CoCl2 can mediate the stabilization of HIF1α protein. Echinomycin is a small-molecule inhibitor of HIF1α, which functions through sequence-specific binding to HRE to competitively inhibit HIF1α binding to its target genes. The m6A sequencing results of the Kasumi-1 cell line treated with CoCl2 and non-treated control were uploaded previously (GSE168778). The differential m6A peaks analysis between CoCl2 + echinomycin group and CoCl2 group was performed.

ORGANISM(S): Homo sapiens

PROVIDER: GSE200605 | GEO | 2022/07/01

REPOSITORIES: GEO