Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Tracheal aspirate bulk RNA sequencing from patients with ARDS [bulk RNA-seq]

ABSTRACT: Trachel aspirates samples were collected from an observational cohort of mechanically ventilated patients to compare respiratory tract gene expression in previous-descried ARDS molecular phenotypes. Patient charts were reviewed by two physicians and patients with ARDS were identified using the Berlin Definition. Control subjects were mechanically ventilated for neurologic indications, were not immunosuppressed, and had no evidence of pulmonary disease. ARDS molecular phenotypes were defined using a previously-described 3-variable classifier (Sinha 2020)

ORGANISM(S): Homo sapiens

PROVIDER: GSE200847 | GEO | 2025/04/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Tracheal aspirate single cell RNA sequencing from patients with ARDS [scRNA-seq]

Project description:Trachel aspirates samples were collected from an observational cohort of mechanically ventilated patients to compare respiratory tract gene expression in previous-descried ARDS molecular phenotypes. Patient charts were reviewed by two physicians and patients with ARDS were identified using the Berlin Definition. ARDS molecular phenotypes were defined using a previously-described 3-variable classifier (Sinha 2020)

2025-04-14 | GSE200848 | GEO

Tracheal aspirate RNA sequencing identifies distinct immunological features of COVID-19 ARDS

Project description:We compared differential gene expression in tracheal aspirates collected mechanically ventilated subjects with COVID-19 ARDS to gene expression in tracheal aspirates from: 1) subjects with ARDS from other casues and 2) mechanically ventilated controls without evidence of pulmonary disease.

2020-12-18 | GSE163426 | GEO

CHOP ARDS Transcriptomic Subtypes (CATS)

Project description:We aimed to identify endotypes of pediatric acute respiratory distress syndrome (ARDS) using whole blood transcriptomics collected within 24 hours of Berlin ARDS onset in intubated children from CHOP Affy microarray and cluster analysis

2020-04-02 | GSE147902 | GEO

Alveolar epithelial glycocalyx degradation mediates surfactant dysfunction and contributes to acute respiratory distress syndrome

Project description:Acute Respiratory Distress Syndrome (ARDS) is a common cause of respiratory failure yet has few pharmacologic therapies, reflecting the mechanistic heterogeneity of lung injury. We hypothesized that damage to the alveolar epithelial glycocalyx, a layer of glycosaminoglycans interposed between the epithelium and surfactant, contributes to lung injury in ARDS patients. Using mass spectrometry of airspace fluid noninvasively collected from mechanically-ventilated patients, we found that airspace glycosaminoglycan shedding (an index of glycocalyx degradation) occurred predominantly in patients with direct lung injury and was associated with duration of mechanical ventilation. Male patients had increased shedding which correlated with airspace concentrations of matrix metalloproteinases. Selective epithelial glycocalyx degradation in mice was sufficient to induce surfactant dysfunction, a key characteristic of ARDS, leading to microatelectasis and decreased lung compliance. Rapid colorimetric quantification of airspace glycosaminoglycans was feasible and could provide point-of-care prognostic information to clinicians and/or be used for predictive enrichment in clinical trials.

2021-12-07 | GSE186705 | GEO

E-GEOD-7041

Project description:Transcription profiling of rat mechanically ventilated lungs from strains resistant or susceptible to ventilation-associated lung injury (VALI)

2014-10-10 | E-GEOD-7041 | ExpressionAtlas

Project description:Airway comparison in mechanically ventilated children

| PRJNA534077 | ENA

3d734370-7e08-44c9-b8ca-ba0c29c086e7 - samples

Project description:Single-cell RNA sequencing of 43 bronchoalveolar lavage fluid (BALF) samples from 15 CAPA and 22 COVID-19 mechanically ventilated patients.

| EGAD00001011342 | EGA

Tracheal aspirate bulk RNA sequencing from patients with ARDS [bulk RNA-seq]

Project description:Tracheal aspirate bulk RNA sequencing from patients with ARDS [bulk RNA-seq]

| PRJNA826911 | ENA

Inhibition of S. pneumoniae by selected sputum samples

Project description:In this experiment several sputum samples from mechanically ventilated patients were tested for their antimicrobial activity. The samples were harvested and digested with trypsin and measured by mass spectrometry.

2020-04-14 | MSV000085288 | MassIVE

Impact of aging on pulmonary cellular responses during mechanical ventilation

Project description:Acute respiratory distress syndrome (ARDS) results in significant morbidity and mortality, especially in the elderly. Mechanical ventilation, a common supportive treatment for ARDS, is necessary for maintaining gas exchange, but can also propagate injury. We hypothesized that aging would exacerbate the pathophysiological responses to mechanical ventilation. Young and aged male mice were mechanically ventilated and changes in surfactant function, inflammation, and vascular permeability were assessed. Additionally, single-cell RNA sequencing was used to delineate cell-specific transcriptional changes. The results showed that surfactant dysfunction was augmented in aged mice, while inflammation was less pronounced in aged animals. Futhermore, vascular permeability was significantly increased with aging. Differential gene expression and pathway analyses revealed that aged endothelial cells exhibited altered cell-cell junction formation and that alveolar macrophages in aged mice showed a blunted inflammatory response. These results highlight the complex interplay between aging and mechanical ventilation, including an age-related predisposition to endothelial barrier dysfunction due to altered cell-cell junction formation and decreased inflammation, potentially due to immune exhaustion. It is concluded that age-related vascular changes may underlie the increased susceptibility to injury during mechanical ventilation in elderly patients.

2025-03-02 | GSE274828 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data