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LPS-induced gene expression in wildtype and MAL knockout mouse macrophages

ABSTRACT: Comparison of gene expression in WT and MAL knockout (MALKO) mouse macrophages treated with 10ng/ml lipopolysaccharide (LPS) with that of mock-treated cells incubated for the same time (10 days). Cells from 4 mice of each genotype were used and each individual served as its own control. Hybridizations of treated and control samples were dye swapped.

ORGANISM(S): Mus musculus

PROVIDER: GSE20091 | GEO | 2010/01/29

SECONDARY ACCESSION(S): PRJNA124175

REPOSITORIES: GEO

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LPS-induced gene expression in wildtype and MAL knockout mouse macrophages

Project description:Comparison of gene expression in WT and MAL knockout (MALKO) mouse macrophages treated with 10ng/ml lipopolysaccharide (LPS) with that of mock-treated cells incubated for the same time (10 days). Cells from 4 mice of each genotype were used and each individual served as its own control. Hybridizations of treated and control samples were dye swapped. Two experiments: WT vs. LPS-treated WT, and MALKO vs. LPS-treated MALKO. 4 individual biological replicates for each genotype, and each mouse served as its own mock control.

2010-01-28 | E-GEOD-20091 | biostudies-arrayexpress

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Project description:Analysis of Bone Marrow derived macrophages (BMDMs) incubated in presence of Lipopolysaccharide (LPS) (10ng/ml) + Interferon -gamma (IFN-g) (20ng/ml) for 8 h vs Mock treated controls

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Effect of LPS and LPS-binding protein treatment for 48 hours on RAW 264.4 cells

Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 48 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 48 hours. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other

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Effect of LPS and LPS-binding protein treatment for 16 hours on RAW 264.4 cells

Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 16 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 16 hours. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other

2004-03-05 | GSE1103 | GEO

Effect of LPS and LPS-binding protein treatment for 8 hours on RAW 264.4 cells

Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 8 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 8 hours. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other

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Effect of LPS and LPS-binding protein treatment for 4 hours on RAW 264.4 cells

Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 4 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 4 hours. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other

2004-03-05 | GSE1101 | GEO

Effect of LPS and LPS-binding protein treatment for 2 hours on RAW 264.4 cells

Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 2 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 2 hours. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other

2004-03-05 | GSE1100 | GEO

Effect of LPS and LPS-binding protein treatment for 1 hour on RAW 264.4 cells

Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for 1 hour. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for 1 hour. Experiment design included three control vs test arrays and three dye swap arrays. Keywords: other

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LPS-induced and E. coli-induced gene expression in wildtype and MyD88-/- mouse macrophages

Project description:Comparison of gene expression in wildtype and MyD88-/- C57BL/6J mouse macrophages treated with 10 ng/mL LPS for 2 hours versus media treated control macrophages, and, wildtype and MyD88-/- C57BL/6J mouse macrophages treated with live E. coli bacteria (log phase; 1 bact per 1 macrophage) for 2 hours versus media treated control macrophages. Cells from 4 mice of each geneotype were used and each individual provided its own control. Hybridizations of treated and control samples from each mouse were dye swap replicated. Wildtype macrophages treated with LPS vs control (GSM22617-GSM22623,GSM22625), MyD88-/- macrophages treated with LPS vs control (GSM22626-GSM22632), wldtype macrophages treated with E. coli vs control (GSM22633-GSM22640, and MyD88-/- macrophages treated with E. coli vs control (GSM22641-GSM22648). Keywords: other

2004-05-16 | GSE1405 | GEO

Time course response of Synechocystis PCC 6803 to dehydration/desiccation

Project description:The responses of the transcriptome were monitored in Synechocysis PCC 6803 during a linear rate of evaporation of the culture to dryness (desiccation). For each time point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each time point) (Custom-commercial array : CyanoCHIP version 2.0, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays. Keywords: Dehydration, stress response, time course, transcription, cyanobacteria

2006-09-04 | GSE4604 | GEO

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