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Genome-Wide Analysis Reveals the Roles of lncRNA in Se accumulation and detoxification in alfalfa (Medicago sativa L.)

ABSTRACT: Medicago sativa L. is the most extensively cultivated and high biomass forage. Selenium is an essential microscale mineral for humans and animals and is a beneficial element for plants, which could increase yields under low concentration and have a poisoning effect under high concentration. LncRNA is involved in diverse biological processes such as flowering, photomorphogenesis, vernalization and stress response in the plants. To study the possible role of lncRNAs in Se accumulation and detoxification underlying alfalfa, we identified and analyzed lncRNAs in alfalfa leaves under 0 mg L-1, 100 mg L-1, 500 mg L-1 based on the degradome deep sequencing. We identified a total of 64,684 novel lncRNAs, from which 3155 were differentially expressed lncRNAs (DELs). The expression patterns of some lncRNAs can be significantly differentially expressed between high and low concentrations. The results of GO and KEGG analysis suggested that sulfate transporter and ABC transporter might be a contributor to Se accumulation in alfalfa. The jasmonic acid (JA) promoted by lipoxygenase and allene oxide synthase may play an important role in Se resistance. The high expression of some key genes of selenocompound metabolism may be beneficial to remove excess Se. The phenylpropanoid, flavonoid, isoflavonoid, and anthocyanidin biosynthesis and autophagy may alleviate the Se toxicity by scavenging reactive oxygen. The expression patterns of nine lncRNAs and their protein-coding genes were validated by qRT-PCR. The same expression pattern was observed between most lncRNAs and their target genes. This study has excavated potential lncRNAs and will give new insight and rich genetic resources to Se-enriched breeding.

ORGANISM(S): Medicago sativa

PROVIDER: GSE200911 | GEO | 2025/04/14

REPOSITORIES: GEO

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Project description:Background: The unprecedented rise in atmospheric CO2 concentration and injudicious fertilization or heterogeneous distribution of Mg in the soil warrant further research to understand the synergistic and holistic mechanisms involved in the plant growth regulation. The objective of this work is to understand responses in plants along with interactive effect of elevated CO2 and Mg levels by comparing data on single stress with that of combined stresses. Results: This study investigated the influence of elevated CO2 (800 μL L−1) on physiological and transcriptomic profiles in Arabidopsis cultured in hydroponic media treated with 1 μM (low), 1000 μM (normal) and 10000 μM (high) Mg2+. Following 7-d treatment, elevated CO2 increased the shoot growth and chlorophyll content under both low and normal Mg supply, whereas root growth was improved exclusively under normal Mg nutrition. Notably, the effect of elevated CO2 on mineral homeostasis in both shoots and roots was less than that of Mg supply. Irrespective of CO2 treatment, high Mg increased leaf number but decreased root growth and absorption of P, K, Ca, Fe and Mn whereas low Mg increased the concentration of P, K, Ca and Fe in leaves. Elevated CO2 decreased the expression of genes related to cadmium response, cell redox homeostasis and lipid localization, but enhanced photosynthesis, signal transduction, protein phosphorylation, NBS-LRR disease resistance proteins and subsequently programmed cell death in low-Mg shoots. By comparison, elevated CO2 enhanced the response of lipid localization (mainly LTP transfer protein/protease inhibitor), endomembrane system, heme binding and cell wall modification in high-Mg roots. Some of these transcriptomic results are substantially in accordance with our physiological and/or biochemical analysis. Conclusions: Contrasting changes were found between roots and shoots with the shoot transcriptome being more severely affected by low Mg while the root transcriptome more affected by high Mg. Elevated CO2 had a greater effect on transcript response in low Mg-fed shoots as well as in high Mg-fed roots. The present findings broaden our current understanding on the interactive effect of elevated CO2 and Mg levels in the Arabidopsis, which may help to design the novel metabolic engineering strategies to cope with Mg deficiency/excess in crops under elevated CO2.

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Genome-Wide Analysis Reveals the Roles of lncRNA in Se accumulation and detoxification in alfalfa (Medicago sativa L.)

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