Similar Datasets
Project description:To understand the role of the SWI/SNF-like ATP-dependent chromatin remodeller SMARCAD1 in pluripotent murine embryonic stem (ES) cells we determined the genome wide binding of triple FLAG tagged SMARCAD1 in PGK12.1 XX ES cells. ChIP was carried out using an antibody against the FLAG-epitope (F1804, Sigma) in double-cross linked chromatin. As controls, input samples and ChIP of PGK12.1 XX ES cells transfected with the empty triple-flag vector were used. The precipitated DNA was subsequently sequenced on an Illumina HiSeq1500.
2019-02-17 | E-MTAB-7014 | biostudies-arrayexpress
Project description:Overexpression of MADS29 results in severely dwarfed phenotype, resulting from a shift in auxin-cytokinin ratio in favor of cytokinins. To see the extent of change in gene expression in the leaves of the transgenic plants, whole genome transcript analysis was carried out using microarray.
2013-09-05 | GSE42028 | GEO
Project description:The effect of the overexpression of Plant Cysteine Oxidase (PCO1) on the transcriptome of Arabidopsis resettes was investigated. For this purpose, 4-week old rosette of wild-type and 35S:FLAG:CDO1 plants were compared. Samples were composed of a pool of 5 plants.
2014-02-07 | GSE44342 | GEO
Project description:The effect of the overexpression of Plant Cysteine Oxidase (PDCO1) on the transcriptome of Arabidopsis resettes was investigated with plants subjected to a 4h hypoxia (5% O2 v/v in air). For this purpose, 4-week old rosette of wild-type and 35S:FLAG:CDO1 plants were compared. Samples were composed of pools of 5 plants.
2014-02-07 | GSE44343 | GEO
Project description:It is important to reveal the regulatory mechanism of OsMYB30 gene which participated in cold response. We used microarrays to find differentially expressed genes that might be regulated by OsMYB30 and resolve its regulation mechanism. The rice gene OsMYB30 was overexpressed in the two OsMYB30 overexpression plants (OE2 and OE28) compared to the control plant (CK, ZH11). The rice gene OsMYB30 was knocked out in the two osmyb30 homozygous mutants (mutant1 and mutant2) compared to the wild type (WT, Huayang).OE2-C0h and OE28-C0h are two repititions of OsMYB30 overexpression plants under normal conditions with CK-C0h used as control plant. OE2-C6h and OE28-C6h are two repititions of OsMYB30 overexpression plants under cold stress conditions with CK-C6h used as control plant. mutant1-C0h and mutant2-C0h are two repititions of osmyb30 homozygous mutant plants under normal conditions with WT-C0h used as control plant. mutant1-C6h and mutant2-C6h are two repititions of osmyb30 homozygous mutant plants under cold stress conditions with WT-C6h used as control plant.
2017-01-20 | GSE83912 | GEO
Project description:TaMYB13 is a transcription factor that has been associated with fructan accumulation in previous studies in wheat (Xue et al. 2011 Plant journal 68: 857 - 870). In this study we aimed to find genes regulated by TaMYB13, through overexpression of this transcription factor in wheat and perform expression analysis by making use of Affymetrix genechip assays. Expression of control plants was compared to transgenic lines, and total RNA was extracted from Flag leaves at anthesis.
2013-07-01 | GSE42000 | GEO
Project description:To understand downstream genes affected by overexpression of OsbHLH148, the Rice 3’-Tiling Microarray analysis (GreenGene Biotech, Yongin, Korea) was carried out to profile gene expression of OsCc1:OsbHLH148 transgenic plants in comparison with wild-type plants under normal growth conditions. RNA samples from these plants were used to generate cyanine-3 (Cy3)-labeled complementary DNA (cDNA) probes, which were then hybridized to the microarray. Each data set was obtained from two biological repeats with independent transgenic lines.
2011-01-01 | GSE22676 | GEO
Project description:In order to assess Tet1 binding, we first generated a Flag tagged Tet1 ES cells and then knocked out Dnmt3a in the [WT, Tet1-Flag] cells. By Tet1 ChIP and Flag ChIP, we showed that Tet1 binding was complementary to Dnmt3a. And Tet1 binding was not affected or slightly increased at majority of its targets.
2018-05-16 | GSE100955 | GEO
Project description:TaMYB13 is a transcription factor that has been associated with fructan accumulation in previous studies in wheat (Xue et al. 2011 Plant journal 68: 857 - 870). In this study we aimed to find genes regulated by TaMYB13, through overexpression of this transcription factor in wheat and perform expression analysis by making use of Affymetrix genechip assays. Expression of control plants was compared to transgenic lines, and total RNA was extracted from Flag leaves at anthesis. In this experiment the genome wide gene expression was assessed from wheat flag leaf material harvested at anthesis. Control plants (BW43,BW44,BW45) were compared with transgenic lines that overexpress TaMYB13 (a20, b2, b36).
2013-07-01 | E-GEOD-42000 | biostudies-arrayexpress
Project description:Overexpression of MADS29 results in severely dwarfed phenotype, resulting from a shift in auxin-cytokinin ratio in favor of cytokinins. To see the extent of change in gene expression in the leaves of the transgenic plants, whole genome transcript analysis was carried out using microarray. Three biological replicates of MADS29 OX (overexpression) lines and Wild Type PB1 (control) plants were used for microarray study
2013-09-05 | E-GEOD-42028 | biostudies-arrayexpress