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Substrates of PRSS35 in PLC cells

ABSTRACT: Since PRSS35 only exhibited tumor suppressive effects in mice, but not in the cultured cells, we speculated that some factors in the microenvironment are affected by PRSS35. To explore this possibility, we performed SILAC proteomic analysis in conjunction with RNA sequencing to identify secreted proteins that decreased in accumulation, but not in transcription level, in the culture medium of PRSS35 overexpressing PLC cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE201311 | GEO | 2023/02/15

REPOSITORIES: GEO

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SILAC proteomic analysis of secreted proteins from PLC cells expressing EV or PRSS35

Project description:Since PRSS35 only exhibited tumor suppressive effects in mice, but not in the cultured cells, we speculated that some factors in the microenvironment are affected by PRSS35. To explore this possibility, we performed SILAC proteomic analysis in conjunction with RNA sequencing to identify secreted proteins that decreased in accumulation, but not in transcription level, in the culture medium of PRSS35 overexpressing PLC cells.

2023-02-15 | PXD033579 | Pride

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Inhibition of the PI-PLC by U73122

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