Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNAseq of WT pMSP3535-PnisA-6his-ftsH(H456Y) and WT pMSP3535-PnisA-6his-ftsH

ABSTRACT: We report the transcriptome of cultures of WT pMSP3535-PnisA-6his-ftsH(H456Y) and WT pMSP3535-PnisA-6his-ftsH following overnight induction with 125 ng/mL nisin

ORGANISM(S): Enterococcus faecalis OG1RF

PROVIDER: GSE201323 | GEO | 2023/08/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

RNAseq to profile transcriptomes in bone marrow-derived macrophages from TKO (mir-106a~363-/- mir-106b~25-/- mir-17~92flox/flox Lyz2-Cre) and WT(Lyz2-Cre) mice stimulated with LPS for 3 hours.

Project description:We performed the transcriptomic analysis of RNA-seq of BMDMs generated from WT and TKO mice and stimulated with 10 ng/ml LPS for 3 h to identify gene expression changes.

2020-03-17 | GSE133844 | GEO

RNA sequencing for enteroids treated with different concentrations of IL-17 (0 ng/ml. 1 ng/ml, 10 ng/ml and 100 ng/ml)

Project description:RNA sequencing for enteroids treated with different concentrations of IL-17 (0 ng/ml. 1 ng/ml, 10 ng/ml and 100 ng/ml)

| PRJNA756286 | ENA

Overexpression analysis of sigZ of B. cereus ATCC 14579.

Project description:The Bacillus cereus ATCC 14579 alternative σ factor σZ and its putative regulon have been characterized. σZ shows overall similarity with ECF σ factors and sigZ constitutes an operon together with asfZ encoding its putative anti-σ factor. Expression analysis revealed sigZ to be induced by an array of stresses, including exposure to ethanol, alkaline pH and heat shock, and a typical promoter binding site for the sigZ-operon was identified by 5’RACE. Phenotypic characterization of B. cereus ATCC 14579 and its sigZ-deletion strain revealed diminished growth performance and sporulation capacity. The σZ-regulon was successfully established by transcriptome analysis of a nisin inducible sigZ-overexpression strain. Overexpression of sigZ was shown to affect expression of 42 genes, including 33 genes encoding proteins located in the extracytoplasm. The identified σZ regulon contained genes encoding proteins situated in the extracytoplasm involved in cell surface modifications and transport. The regulation of genes encoding cell surface modification proteins implies σZ to be involved in the regulation of interaction of B. cereus ATCC 14579 with its environments, which includes human intestinal cells, possibly influencing its virulence status. Both an empty vector strain and an sigZ overexpressing strain were grown to an OD600 of ~0.5, after which 0.2 ng/ml nisin was added to both cultures. After 90 minutes from both cultures a sample was taken for RNA isolation. Comparisons performed were in duplicate with dye swap, the empty vector strain after 90 min nisin with the overexpressing strain after 90 min nisin.

2008-05-10 | E-GEOD-9860 | biostudies-arrayexpress

Bulk RNAseq of AML and endothelial cells in untreated and cytokine-treated conditions

Project description:We report the use of bulk RNAseq of AML and endothelial cell lines in four conditions (1. Untreated; 2. IFNg - 10 ng/ml; 3. TNFa - 10 ng/ml; 4. IFNg and TNFa - 10 ng/ml) in order to identify genes that are differentially expressed between the two cell types, both at baseline and in an inflammatory microenvironment.

2021-10-21 | GSE159991 | GEO

Genome-wide analysis of the role of S276 phosphorylation of p65-NFkappaB on the regulation of gene expression in response to EGF or IL-1 treatment.

Project description:Genome-wide gene expression levels of were measured by microarray in wildtype (WT) or S276A-p65 HMECs treated with or without 100 ng/mL EGF or 10 ng/mL IL-1 for 4 h.

2018-08-31 | GSE73914 | GEO

TGF-ß treatment of human melanoma cell lines

Project description:To analyse whether the activation of TGF-ß signalling (recombinant hTGF-ß, 5 ng/mL; PeproTech) is able to invoke a full phenotype- switch, we performed RNA sequencing of melanoma cultures from two independent melanoma cell lines at two different time point after TGF-β1 (5 ng/ml, preprotech).

2020-04-17 | GSE148767 | GEO

Dose dependent reprogramming of neutrophils by LPS

Project description:We have performed analyses of murine primary bone marrow derived neutrophils challenged with either ultra-low dose or high dose of LPS. Neutrophils can be differentially programmed to distinct states by varying dosages of LPS. Purified bone marrow neutrophils were treated with PBS, 100 pg/ml LPS or 100 ng/ml LPS overnight, and harvested for scRNAseq analysis to examine their profiles of gene expression.

2023-12-27 | GSE230241 | GEO

RNA Sequencing data of gene expression chenges induced by NGPF2 treatment in primary neuronal culture

Project description:Mouse cortical primary neuronal cultures (DIV12-13) were treated with NGPF2 (1.5 ng/ml) for 2 hours and then fixed for RNA sequencing.

2021-07-06 | GSE179402 | GEO

HSP27 3A vs WT

Project description:human bronchial smooth muscle cells were infected with adenoviral constructs containing either the phosphorylation mutant form of HSP27 (HSP27-3A) or the wild type form (HSP27-WT) at 40 MOI for 4 days. All sets were stimulated with a cytokine mixture containing 10 ng/ml IL-1beta, TNF-alfa, and gamma-IFN for 20 hours prior to harvest. This series represents 4 separate preparations of cells and RNA, with each set being subjected to a dye flip and hybridized to 2 different slides. Keywords: parallel sample

2004-04-09 | GSE1292 | GEO

TGFbeta lung fibroblasts

Project description:The response to a 4 hour treatment with TGFbeta (4 ng/ml) was evaluated in lung fibroblasts derived from three controls (normal periphery of resected tumor), open lung biopsies from three patients with idiopathic pulmonary fibrosis (usual interstitial pneumonia pattern on biopsy) and from three patients with fibrosing alveolitis associated with systemic sclerosis (fibrotic non specific interstitial pneumonia pattern on biopsy). Lung fibroblasts were grown to confluence in DMEM with 10% fetal calf serum. At confluence, lung fibroblasts were serum-deprived overnight, and exposed to either 4 ng/ml of activated TGF-ß1 (R&D Systems) or serum-free culture medium with 0.1% BSA for four hours. Keywords: other

2004-11-02 | GSE1724 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data