Transcriptomics,Genomics

Dataset Information

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Analysis of genes in HNSCC cells regulated following exposure to cisplatin and involved in tumourigenesis


ABSTRACT: Analysis of genes regulated by cisplatin exposure, using non-tumourigenic keratinocytes to filter out genes regulated following cisplatin exposure that are unlikely to be involved in tumourigenesis. Results identify genes that may be important in mediating an increase in tumourigenic potential in cisplatin-resistant HNSCC cells. Overall design: Where indicated, cisplatin (CDDP)-treated cells were incubated after treatment to allow susceptible cells to undergo cell death. Two time points were used to investigate earlier and later gene expression changes following cisplatin treatment. The FaDu and Detroit-562 cell lines were sorted at the indicated times to obtain the smallest 10-20% of cells as these had been previously found to be the cells with the highest viability following treatment. Total RNA was obtained from head and neck squamous cell carcinoma (HNSCC) cells (FaDu, Detroit-562) at 2 timepoints subsequent to cisplatin treatment, and compared to untreated control cells. Genes differentially expressed in the same direction in non-tumourigenic human epidermal keratinocytes (HEKs) were excluded from subsequent analyses as these were deemed unlikely to be involved in mediating tumourigenic potential. There were a total of 9 conditions analysed, with 3 biological replicates for each, for a total of 27 samples. The supplementary file 'GSE20185_non-normalized.txt' contains non-normalized data for Samples GSM506367-GSM506393.

INSTRUMENT(S): Illumina HumanHT-12 V3.0 expression beadchip

SUBMITTER: Kim Poth   

PROVIDER: GSE20185 | GEO | 2010-06-26

SECONDARY ACCESSION(S): PRJNA125677

REPOSITORIES: GEO

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Publications

Cisplatin treatment induces a transient increase in tumorigenic potential associated with high interleukin-6 expression in head and neck squamous cell carcinoma.

Poth Kim J KJ   Guminski Alexander D AD   Thomas Gethin P GP   Leo Paul J PJ   Jabbar Ibtissam A IA   Saunders Nicholas A NA  

Molecular cancer therapeutics 20100803 8


Head and neck squamous cell carcinoma (HNSCC) is characterized by the 5-year survival rate of approximately 50%. Despite aggressive surgical, radiation, and chemotherapeutic interventions, 30% to 40% of patients die from the development of recurrent or disseminated disease that is resistant to chemotherapy. As a model of recurrence, we examined the effects of cisplatin on the ability of head and neck cancer cells to initiate tumors in a xenotransplant model. HNSCC cells were treated in vitro wit  ...[more]

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