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Profiles of Differentially Expressed Long-Noncoding RNAs and mRNAs in Non-alcoholic Fatty Liver Disease Mice

ABSTRACT: Non-coding RNAs (ncRNAs), including piwi-interacting RNA (piRNAs), circular RNAs (circRNAs) and long ncRNAs (lncRNAs), have been recently emerged as key regulators of pathological process of metabolic diseases. Since previous studies have reported that piRNAs and circRNAs involve in non-alcoholic fatty liver disease (NAFLD), this study was designed to explore more potential regulators of lncRNAs in NAFLD. A NAFLD mouse model was successfully constructed by providing high-fat diet (HFD) for 16 weeks as determined by immunohistology and serology assay. The lncRNAs expression profile including 549 upregulated lncRNAs and 108 downregulated lncRNAs was observed using microarray analysis, which was further confirmed by quantitative real-time Polymerase Chain Reaction (qRT-PCR). Moreover, we identified 308 differentially expressed mRNAs in the NAFLD group. Importantly, the sphingolipid signaling pathway may be significantly enriched pathways closely related to NAFLD using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Further study revealed that lncRNAs and their interacting mRNAs may participate in the pathophysiological process of NAFLD by regulating sphingolipid signaling pathway. Taken together, the current study demonstrated more potential regulators of lncRNAs and the interacting pathways between lncRNAs and mRNAs, which may provide new insights into the pathogenesis of NAFLD.

ORGANISM(S): Mus musculus

PROVIDER: GSE202035 | GEO | 2022/05/03

REPOSITORIES: GEO

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