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Transcriptional analysis of quorum sensing null strain in Yersinia pestis CO92 at 30°C

ABSTRACT: Yersinia pestis is the etiology of plague that is able to sense cell density by quorum sensing. The function of quorum sensing in Y.pestis is not clear. Here, the process of quorum sensing was investigated by comparing transcript profiles when three quorum sensing synthase genes are knocked out. Two strains, ∆pgm (pigmentation-negative) mutant R88 as treatment and 3XQS mutant with mutation (∆pgm, ∆ypeIR, ∆yspIR, and ∆luxS) R115 as control are used in this analysis.

ORGANISM(S): Yersinia pestis

PROVIDER: GSE20217 | GEO | 2016/01/01

SECONDARY ACCESSION(S): PRJNA153901

REPOSITORIES: GEO

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Transcriptional analysis of quorum sensing deficient in Yersinia pestis CO92 at 37°C

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Transcriptional analysis of quorum sensing null strain in Yersinia pestis CO92 at 30Â°C

Project description:Yersinia pestis is the etiology of plague that is able to sense cell density by quorum sensing. The function of quorum sensing in Y.pestis is not clear. Here, the process of quorum sensing was investigated by comparing transcript profiles when three quorum sensing synthase genes are knocked out. Two strains, â??pgm (pigmentation-negative) mutant R88 as treatment and 3XQS mutant with mutation (â??pgm, â??ypeIR, â??yspIR, and â??luxS) R115 as control are used in this analysis. Six independent RNA samples from R115 cultures were paired with six independent RNA samples from R88 cultures for hybridization to six two-color microarrays. Dye-swap design was used to remove the Cy5 and Cy3 dye bias.

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Transcriptional analysis of AHLs signals add in experiment inYersinia pestis CO92 at 37°C

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Transcriptional analysis of three quorum sensing signals add in experiment inYersinia pestis CO92 at 37°C

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2016-07-03 | E-GEOD-22847 | biostudies-arrayexpress

Global gene expression analysis of Yersinia pestis Ype AHL quorum sensing at 30Â°C

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