RNA profiles of polysomes from MOLM-13 cells treated with FLT3 inhibitor and/or mTOR inhibitor in the presence of conditioned media of bone marrow stomal cells
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ABSTRACT: RNA-seq analysis of MOLM13 cells to compare mRNAs enriched in heavy polysomes post FLT3 inhibition and/or mTOR inhibition in the presence or absence of conditioned media of bone marrow stromal cells HS-5 (hBMSC-CM)
Project description:RNA-seq analysis of MOLM-13 cells to compare the gene expression profiles post FLT3 inhibition and/or ATM inhibition in the presence or absence of conditioned media of bone marrow stromal cell HS-5
Project description:RNA profiles of polysomes from MOLM-13 cells treated with FLT3 inhibitor and/or mTOR inhibitor in the presence of conditioned media of bone marrow stomal cells
Project description:Translational profiles of wild type yeast strains were identified by microarray analysis by isolating polysomes using sucrose gradients to recover polysome-associated RNA and comparing polysome-associated RNA vs total RNA
Project description:We performed ribosome profiling of polysomes (Poly-RIBOseq) to monitor translation of RNAs associated with polysomes in brain extracts from a mouse model Keywords: ribosome profiling, translation, polysome profiling, Poly-RIBOseq
Project description:Translational profiles of wild type yeast strains were identified by microarray analysis by isolating polysomes using sucrose gradients to recover polysome-associated RNA and comparing polysome-associated RNA vs total RNA Set of arrays that are part of repeated experiments
Project description:Molm13 AML cell line both control and MPI KO, treated with AC220 (quizartinib) and mannose at 24 and 72 hours after drug treatment. 2 reads and 2 repeats for each condition, 2 timepoints, 48 total files.
Project description:RNA-seq followed by digital gene expression analyses we assessed genes that change upon CRISPR-Cas9-mediated knockout of YBX1 in myeloid leukemia cells (MOLM13 cell line).
Project description:To gain a global view of translational inhibition by microRNA (miRNA), we isolated polysomes from wild type and Dicer1 knockout HCT116 human cells using sucrose gradient fractionation technique. The polysome fraction was separated into light (9mer or less) and heavy (10mer or more) subfractions. RNA samples were extracted from both subfractions and subjected to RNA-seq analysis. A general shift from light to heavy subfractions was detected for miRNA targted mRNAs.